{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T11:55:18Z","timestamp":1777463718419,"version":"3.51.4"},"reference-count":34,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2014,2,1]],"date-time":"2014-02-01T00:00:00Z","timestamp":1391212800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Chem Biol Drug Des"],"published-print":{"date-parts":[[2014,5]]},"abstract":"<jats:p>Ribavirin and lamivudine are representatives of antiviral drugs that are widely used to treat viral infections, especially chronic liver disease. To compare binding mechanism and behavior of antiviral drugs with human serum albumin (<jats:styled-content style=\"fixed-case\">HSA<\/jats:styled-content>), we performed fluorescence spectroscopy and X\u2010ray crystallography to investigate the interactions of ribavirin and lamivudine with<jats:styled-content style=\"fixed-case\">HSA<\/jats:styled-content>. Fluorescence spectroscopy showed ribavirin and lamivudine inhibit binding affinity each other. Our results further demonstrated that ribavirin and lamivdudine interaction with<jats:styled-content style=\"fixed-case\">HSA<\/jats:styled-content>could be affected by the presence of other compounds, including the non\u2010steroidal anti\u2010inflammatory drugs, indometacin. X\u2010ray structures revealed that ribavirin and lamivudine bind in<jats:styled-content style=\"fixed-case\">IIA<\/jats:styled-content>subdomain of<jats:styled-content style=\"fixed-case\">HSA<\/jats:styled-content>mainly by forming hydrogen bond and hydrophobic interactions forces. The carboxamido of ribavirin forms hydrogen bonds with Arg222; Hydroxyl group (6) of ribavirin forms hydrogen bond with Arg257. Hydroxyl group (15) of lamivudine forms hydrogen bond with Arg222; amino group (4) of lamivudine forms hydrogen bond with carbonyl of Arg257. Our results reveal the key biochemical and structural characteristics of the<jats:styled-content style=\"fixed-case\">HSA<\/jats:styled-content>interaction with ribavirin and lamivudine, providing guidance for future development of ribavirin\u2010 and lamivudine\u2010based compounds and a drug\u2010<jats:styled-content style=\"fixed-case\">HSA<\/jats:styled-content>delivery system.<\/jats:p>","DOI":"10.1111\/cbdd.12270","type":"journal-article","created":{"date-parts":[[2013,12,10]],"date-time":"2013-12-10T16:20:11Z","timestamp":1386692411000},"page":"576-582","source":"Crossref","is-referenced-by-count":12,"title":["Comparison of Binding Characterization of Two Antiviral Drugs to Human Serum Albumin"],"prefix":"10.1111","volume":"83","author":[{"given":"Mei","family":"Li","sequence":"first","affiliation":[{"name":"State Key Laboratory Cultivation Base for the Chemistry and Molecular Engineering of Medicinal Resources Ministry of Science and Technology of China Guangxi Normal University Guilin 541004 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Erin","family":"McAuley","sequence":"additional","affiliation":[{"name":"Ben May Department for Cancer Research University of Chicago Chicago IL 60637 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yao","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory Cultivation Base for the Chemistry and Molecular Engineering of Medicinal Resources Ministry of Science and Technology of China Guangxi Normal University Guilin 541004 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"LinLin","family":"Kong","sequence":"additional","affiliation":[{"name":"State Key Laboratory Cultivation Base for the Chemistry and Molecular Engineering of Medicinal Resources Ministry of Science and Technology of China Guangxi Normal University Guilin 541004 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Feng","family":"Yang","sequence":"additional","affiliation":[{"name":"State Key Laboratory Cultivation Base for the Chemistry and Molecular Engineering of Medicinal Resources Ministry of Science and Technology of China Guangxi Normal University Guilin 541004 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"ZuPing","family":"Zhou","sequence":"additional","affiliation":[{"name":"State Key Laboratory Cultivation Base for the Chemistry and Molecular Engineering of Medicinal Resources Ministry of Science and Technology of China Guangxi Normal University Guilin 541004 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"XiaoYang","family":"Wu","sequence":"additional","affiliation":[{"name":"Ben May Department for Cancer Research University of Chicago Chicago IL 60637 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong","family":"Liang","sequence":"additional","affiliation":[{"name":"State Key Laboratory Cultivation Base for the Chemistry and Molecular Engineering of Medicinal Resources Ministry of Science and Technology of China Guangxi Normal University Guilin 541004 China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,2]]},"reference":[{"key":"e_1_2_7_2_1","volume-title":"All About Albumin: Biochemistry, Genetics, and Medical Applications","author":"Peters T.","year":"1995"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2013.05.005"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1080\/15216540500404093"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0436576100"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2013.06.018"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2005.07.075"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mam.2011.12.002"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0506440102"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/358209a0"},{"key":"e_1_2_7_11_1","doi-asserted-by":"crossref","first-page":"824","DOI":"10.1016\/S0026-895X(25)10691-3","article-title":"The characterization of two specific drug binding sites on human serum albumin","volume":"11","author":"Sudlow G.","year":"1975","journal-title":"Mol Pharmacol"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja908878n"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.2133\/dmpk.24.342"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2013.06.032"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2008.06.016"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1166\/jbn.2013.1608"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0033-1345718"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1517\/17425247.2.4.707"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2011.09.069"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2011.11.028"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2008.05.010"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1517\/17425247.2010.498474"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/1869"},{"key":"e_1_2_7_24_1","volume-title":"The PyMol Molecular Graphics System","author":"DeLano W.L.","year":"2004"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0037468"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2004.06.021"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0008834"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja8031289"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.2000.4158"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0036723"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhazmat.2013.01.036"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsb.2006.08.015"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2042-7158.1996.tb03990.x"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsb.2007.12.004"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1021\/mp300322y"}],"container-title":["Chemical Biology &amp; Drug Design"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fcbdd.12270","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cbdd.12270","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T03:59:26Z","timestamp":1746071966000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/cbdd.12270"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,2]]},"references-count":34,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2014,5]]}},"alternative-id":["10.1111\/cbdd.12270"],"URL":"https:\/\/doi.org\/10.1111\/cbdd.12270","archive":["Portico"],"relation":{},"ISSN":["1747-0277","1747-0285"],"issn-type":[{"value":"1747-0277","type":"print"},{"value":"1747-0285","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,2]]}}}