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Antimycobacterial activity was determined against <jats:italic>Mycobacterium tuberculosis<\/jats:italic> H37Rv, <jats:italic>M.\u00a0kansasii<\/jats:italic> and two strains of <jats:italic>M.\u00a0avium<\/jats:italic>. Generally, the antimycobacterial activity increased with prolongation of simple alkyl chain and culminated in compounds with heptylamino substitution (<jats:bold>3e<\/jats:bold>,<jats:bold> 4e<\/jats:bold>) with <jats:styled-content style=\"fixed-case\">MIC<\/jats:styled-content>\u00a0=\u00a05\u201310\u00a0\u03bc<jats:sc>m<\/jats:sc> against <jats:italic>M.\u00a0tuberculosis<\/jats:italic> H37Rv. On the contrary, derivatives with modified alkyl chain (containing e.g. terminal methoxy or hydroxy group) as well as phenylalkylamino derivatives were mainly inactive. The most active compounds (with hexyl to octylamino substitution) were evaluated for their <jats:italic>in vitro<\/jats:italic> activity against drug\u2010resistant strains of <jats:italic>M.\u00a0tuberculosis<\/jats:italic> and possessed activity comparable to that of the reference drug isoniazid. None of the tested compounds were active against <jats:italic>M.\u00a0avium<\/jats:italic>. Some derivatives exhibited activity against Gram\u2010positive bacteria including methicillin\u2010resistant <jats:italic>Staphylococcus aureus<\/jats:italic> (best <jats:styled-content style=\"fixed-case\">MIC<\/jats:styled-content>\u00a0=\u00a07.8\u00a0\u03bc<jats:sc>m<\/jats:sc>), while Gram\u2010negative strains as well as tested fungal strains were completely unsusceptible. Active compounds were tested for <jats:italic>in vitro<\/jats:italic> toxicity on various cell lines and in most cases were non\u2010toxic up to 100\u00a0\u03bc<jats:sc>m<\/jats:sc>.<\/jats:p>","DOI":"10.1111\/cbdd.12536","type":"journal-article","created":{"date-parts":[[2015,2,12]],"date-time":"2015-02-12T18:26:01Z","timestamp":1423765561000},"page":"674-681","source":"Crossref","is-referenced-by-count":8,"title":["Synthesis and Antimicrobial Evaluation of 6\u2010Alkylamino\u2010<i>N<\/i>\u2010phenylpyrazine\u20102\u2010carboxamides"],"prefix":"10.1111","volume":"86","author":[{"given":"Barbora","family":"Servusova\u2010Vanaskova","sequence":"first","affiliation":[{"name":"Faculty of Pharmacy in Hradec Kr\u00e1lov\u00e9 Charles University in Prague Heyrovsk\u00e9ho 1203 Hradec Kr\u00e1lov\u00e9 500 05 Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pavla","family":"Paterova","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy in Hradec Kr\u00e1lov\u00e9 Charles University in Prague Heyrovsk\u00e9ho 1203 Hradec Kr\u00e1lov\u00e9 500 05 Czech Republic"},{"name":"Department of Clinical Microbiology University Hospital Hradec Kr\u00e1lov\u00e9 Sokolsk\u00e1 581 Hradec Kr\u00e1lov\u00e9 500 05 Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vladimir","family":"Garaj","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry Faculty of Pharmacy Comenius University Odboj\u00e1rov 10 Bratislava 832 32 Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jana","family":"Mandikova","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy in Hradec Kr\u00e1lov\u00e9 Charles University in Prague Heyrovsk\u00e9ho 1203 Hradec Kr\u00e1lov\u00e9 500 05 Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiri","family":"Kunes","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy in Hradec Kr\u00e1lov\u00e9 Charles University in Prague Heyrovsk\u00e9ho 1203 Hradec Kr\u00e1lov\u00e9 500 05 Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lieve","family":"Naesens","sequence":"additional","affiliation":[{"name":"Rega Institute for Medical Research KU Leuven Minderbroedersstraat 10 Leuven 3000 Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Petr","family":"J\u00edlek","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy in Hradec Kr\u00e1lov\u00e9 Charles University in Prague Heyrovsk\u00e9ho 1203 Hradec Kr\u00e1lov\u00e9 500 05 Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin","family":"Dolezal","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy in Hradec Kr\u00e1lov\u00e9 Charles University in Prague Heyrovsk\u00e9ho 1203 Hradec Kr\u00e1lov\u00e9 500 05 Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan","family":"Zitko","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy in Hradec Kr\u00e1lov\u00e9 Charles University in Prague Heyrovsk\u00e9ho 1203 Hradec Kr\u00e1lov\u00e9 500 05 Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,3,6]]},"reference":[{"key":"e_1_2_8_2_1","unstructured":"World Health Organization.Global Tuberculosis Report 2014. 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