{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,4]],"date-time":"2026-04-04T10:07:20Z","timestamp":1775297240493,"version":"3.50.1"},"reference-count":31,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2015,4,7]],"date-time":"2015-04-07T00:00:00Z","timestamp":1428364800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100010892","name":"Department of science and Technology","doi-asserted-by":"crossref","award":["SR\/FT\/CS-61\/2010"],"award-info":[{"award-number":["SR\/FT\/CS-61\/2010"]}],"id":[{"id":"10.13039\/501100010892","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Chem Biol Drug Des"],"published-print":{"date-parts":[[2015,10]]},"abstract":"<jats:p>We investigated the antifungal potential of short lipopeptides against clinical fungal isolates with an objective to evaluate their clinical feasibility. All tested lipopeptides exhibit good antifungal activity with negligible difference between the <jats:styled-content style=\"fixed-case\">MIC<\/jats:styled-content>s against susceptible and drug\u2010resistant clinical fungal isolates. The <jats:styled-content style=\"fixed-case\">MTT<\/jats:styled-content> assay results revealed the lower cytotoxicity of lipopeptides toward mammalian cells (<jats:styled-content style=\"fixed-case\">NRK<\/jats:styled-content>\u201052E). In particular, <jats:styled-content style=\"fixed-case\">LP<\/jats:styled-content>24 displayed highest potency against most of the tested fungal isolates with <jats:styled-content style=\"fixed-case\">MIC<\/jats:styled-content>s in the range of 1.5\u20134.5\u00a0<jats:italic>\u03bc<\/jats:italic>g\/mL. Calcein dye leakage experiments with model membrane suggested the membrane\u2010active mode of action for <jats:styled-content style=\"fixed-case\">LP<\/jats:styled-content>24. Extending our work from model membranes to intact <jats:italic>Aspergillus fumigatus<\/jats:italic> in scanning electron micrographs, we could visualize surface perturbation caused by <jats:styled-content style=\"fixed-case\">LP<\/jats:styled-content>24. <jats:styled-content style=\"fixed-case\">LP<\/jats:styled-content>24 (5\u00a0mg\/kg) significantly reduces the <jats:italic>A.\u00a0fumigatus<\/jats:italic> burden among the various organs of infected animals, and 70% of the infected mice survived when observed for 28\u00a0days. This study underscores the potential of small cationic abiotic lipopeptides to develop into the next\u2010generation antimicrobial therapy.<\/jats:p>","DOI":"10.1111\/cbdd.12558","type":"journal-article","created":{"date-parts":[[2015,3,17]],"date-time":"2015-03-17T00:50:54Z","timestamp":1426553454000},"page":"829-836","source":"Crossref","is-referenced-by-count":3,"title":["<i>In Vitro<\/i> and <i>In Vivo<\/i> Evaluation of Small Cationic Abiotic Lipopeptides as Novel Antifungal Agents"],"prefix":"10.1111","volume":"86","author":[{"given":"Sandeep","family":"Lohan","sequence":"first","affiliation":[{"name":"Department of Pharmacy Jaypee University of Information Technology Solan 173234 India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jitender","family":"Monga","sequence":"additional","affiliation":[{"name":"Department of Urology Postgraduate Institute of Medical Education and Research Chandigarh 160012 India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chetan Singh","family":"Chauhan","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Bhupal Noble College of Pharmacy Udaipur Rajasthan 313002 India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gopal Singh","family":"Bisht","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Jaypee University of Information Technology Solan 173234 India"},{"name":"Department of Biotechnology and Bioinformatics Jaypee University of Information Technology Solan 173234 India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,4,7]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.4103\/2141-9248.113669"},{"key":"e_1_2_8_3_1","first-page":"39","article-title":"Clinical characteristics and outcomes in 303 HIV\u2010infected patients with invasive fungal infections: data from the prospective antifungal therapy alliance registry, a multicenter, observational study","volume":"6","author":"Marukutira T.","year":"2014","journal-title":"HIV AIDS (Auckl)"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0507.2010.01961.x"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/tid.12197"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmcl.2014.04.120"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2007.03.009"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.2174\/092986706776873023"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1086\/344658"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1021\/ml500011v"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1039\/c4md00041b"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/415389a"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-011-0710-x"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1094\/MPMI-19-0699"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0282(1997)43:1<15::AID-BIP3>3.0.CO;2-3"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(03)14472-8"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkg117"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0606129103"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi011549t"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi035142v"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M312260200"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20050520"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi8011675"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/cbdd.12182"},{"key":"e_1_2_8_25_1","volume-title":"Reference Method for Broth Dilution Antifungal Susceptibility Testing of Filamentous Fungi; Approved Standard","author":"Clinical and Laboratory Standards Institute","year":"2008"},{"key":"e_1_2_8_26_1","volume-title":"Reference Method for Broth Dilution Antifungal Susceptibility Testing of Yeasts; Approved Standard M27\u2010A3","author":"Clinical and Laboratory Standards Institute","year":"2008"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2014.06.023"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.2650090211"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.mi.48.100194.002351"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(95)80370-X"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1747-0285.2010.00973.x"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2008.06.018"}],"container-title":["Chemical Biology &amp; Drug Design"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fcbdd.12558","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cbdd.12558","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T20:01:37Z","timestamp":1696363297000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/cbdd.12558"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,4,7]]},"references-count":31,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2015,10]]}},"alternative-id":["10.1111\/cbdd.12558"],"URL":"https:\/\/doi.org\/10.1111\/cbdd.12558","archive":["Portico"],"relation":{},"ISSN":["1747-0277","1747-0285"],"issn-type":[{"value":"1747-0277","type":"print"},{"value":"1747-0285","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,4,7]]}}}