{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T13:52:04Z","timestamp":1772805124021,"version":"3.50.1"},"reference-count":34,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2015,4,7]],"date-time":"2015-04-07T00:00:00Z","timestamp":1428364800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Chem Biol Drug Des"],"published-print":{"date-parts":[[2015,10]]},"abstract":"<jats:p>Carbonic anhydrases (<jats:styled-content style=\"fixed-case\">CA<\/jats:styled-content>s) are known as a drug\u2013target enzymes. The inhibitors of the enzyme are important compounds for discovering new therapeutic agents and understanding in detail protein\u2013drug interactions at the molecular level. For this purpose, the <jats:italic>in\u00a0vitro<\/jats:italic> effects of some anti\u2010inflammatory agents such as tenoxicam, fluorometholone acetate, and dexamethasone were investigated on esterase activity of human erythrocyte <jats:styled-content style=\"fixed-case\">CA<\/jats:styled-content>\u2010I and <jats:styled-content style=\"fixed-case\">CA<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">II<\/jats:styled-content> in this study. <jats:styled-content style=\"fixed-case\">hCA<\/jats:styled-content>\u2010I and <jats:styled-content style=\"fixed-case\">hCA<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">II<\/jats:styled-content> were purified by affinity chromatography with a yield of 47.25% and 87%, and a specific activity of 642.8\u00a0EU\/mg proteins and 5576.9\u00a0EU\/mg proteins, respectively. <jats:styled-content style=\"fixed-case\">SDS<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PAGE<\/jats:styled-content> was performed to determine the purity of the enzymes. Inhibitory effects of the drugs on <jats:styled-content style=\"fixed-case\">hCA<\/jats:styled-content>\u2010I and <jats:styled-content style=\"fixed-case\">hCA<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">II<\/jats:styled-content> were determined by spectrophotometric method. <jats:italic><jats:styled-content style=\"fixed-case\">IC<\/jats:styled-content><\/jats:italic><jats:sub>50<\/jats:sub> values for <jats:styled-content style=\"fixed-case\">hCA<\/jats:styled-content>\u2010I and <jats:styled-content style=\"fixed-case\">hCA<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">II<\/jats:styled-content> were 0.198, 2.18, 11.7, 0.11, 17.5 and 14\u00a0<jats:italic>\u03bc<\/jats:italic><jats:sc>m<\/jats:sc> using tenoxicam, fluorometholone acetate, and dexamethasone, respectively. For fluorometholone acetate and dexamethasone, <jats:italic>K<\/jats:italic><jats:sub>i<\/jats:sub> values from Lineweaver\u2013Burk plots were obtained as 1.044 and 21.2\u00a0<jats:italic>\u03bc<\/jats:italic><jats:sc>m<\/jats:sc> (noncompetitive) for <jats:styled-content style=\"fixed-case\">hCA<\/jats:styled-content>\u2010I and 9.98 and 8.66\u00a0<jats:italic>\u03bc<\/jats:italic><jats:sc>m<\/jats:sc> (non\u2010competitive) for <jats:styled-content style=\"fixed-case\">hCA<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">II<\/jats:styled-content>. In conclusion, tenoxicam, fluorometholone acetate, and dexamethasone showed potent inhibitory effects on esterase activity of <jats:styled-content style=\"fixed-case\">hCA<\/jats:styled-content>\u2010I and <jats:styled-content style=\"fixed-case\">hCA<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">II<\/jats:styled-content> isozymes under <jats:italic>in\u00a0vitro<\/jats:italic> conditions.<\/jats:p>","DOI":"10.1111\/cbdd.12561","type":"journal-article","created":{"date-parts":[[2015,3,24]],"date-time":"2015-03-24T20:53:28Z","timestamp":1427230408000},"page":"857-863","source":"Crossref","is-referenced-by-count":35,"title":["Some Anti\u2010Inflammatory Agents Inhibit Esterase Activities of Human Carbonic Anhydrase Isoforms I and <scp>II<\/scp>: An <i>In Vitro<\/i> Study"],"prefix":"10.1111","volume":"86","author":[{"given":"Zuhal","family":"Al\u0131m","sequence":"first","affiliation":[{"name":"Department of Chemistry Faculty of Sciences Atat\u00fcrk University 25240 Erzurum Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nam\u0131k","family":"K\u0131l\u0131n\u00e7","sequence":"additional","affiliation":[{"name":"Department of Chemistry Faculty of Sciences Atat\u00fcrk University 25240 Erzurum Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mehmet M.","family":"\u0130\u015fg\u00f6r","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Faculty of Veterinary Sciences Mustafa Kemal University 31000 Hatay Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B\u00fclent","family":"\u015eeng\u00fcl","sequence":"additional","affiliation":[{"name":"Department of Chemistry Faculty of Sciences Atat\u00fcrk University 25240 Erzurum Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u015e\u00fckr\u00fc","family":"Beydemir","sequence":"additional","affiliation":[{"name":"Department of Chemistry Faculty of Sciences Atat\u00fcrk University 25240 Erzurum Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,4,7]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd2467"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1021\/jm901524f"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/9780470508169.ch5"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.3109\/14756366.2011.614607"},{"key":"e_1_2_5_6_1","first-page":"580","article-title":"Some drugs inhibit in\u00a0vitro hydratase and esterase activities of human carbonic anhydrase\u2010I and II","volume":"59","author":"Ekinci D.","year":"2007","journal-title":"Pharmacol Rep"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.3109\/14756366.2011.651465"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmc.2012.11.012"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1136\/bjo.2007.125187"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0920-1211(03)00078-0"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinbiochem.2008.06.017"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1080\/14756360802608351"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2009.06.048"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12011-009-8500-0"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2010.07.030"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharep.2013.08.007"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.3109\/13813455.2012.688055"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmc.2009.07.019"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.2165\/00003088-199426010-00003"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.2165\/00003495-199141040-00008"},{"key":"e_1_2_5_21_1","volume-title":"Rasyonel Tedavi Y\u00f6n\u00fcnden Tibbi, Farmakoloji","author":"Kayaalp O.","year":"2000"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1006\/phrs.2000.0674"},{"key":"e_1_2_5_23_1","doi-asserted-by":"crossref","first-page":"1065","DOI":"10.1016\/S0021-9258(18)91392-X","article-title":"Carbonic anhydrase from human erythrocytes","volume":"239","author":"Rickli E.E.","year":"1964","journal-title":"J Biol Chem"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)51011-5"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)95800-X"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2007.00901.x"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3040.2001.00669.x"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jphotobiol.2011.01.021"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.3109\/14756366.2012.672983"},{"key":"e_1_2_5_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmc.2012.04.044"},{"key":"e_1_2_5_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1747-0285.2011.01104.x"},{"key":"e_1_2_5_33_1","doi-asserted-by":"publisher","DOI":"10.4103\/1319-2442.111067"},{"key":"e_1_2_5_34_1","first-page":"787","article-title":"Glucose 6\u2010phosphate dehydrogenase: In vitro and in\u00a0vivo effects of dantrolene sodium","volume":"55","author":"Beydemir \u015e.","year":"2003","journal-title":"Pol J Pharmacol"},{"key":"e_1_2_5_35_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11099-005-1011-0"}],"container-title":["Chemical Biology &amp; Drug Design"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fcbdd.12561","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cbdd.12561","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T20:01:02Z","timestamp":1696363262000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/cbdd.12561"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,4,7]]},"references-count":34,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2015,10]]}},"alternative-id":["10.1111\/cbdd.12561"],"URL":"https:\/\/doi.org\/10.1111\/cbdd.12561","archive":["Portico"],"relation":{},"ISSN":["1747-0277","1747-0285"],"issn-type":[{"value":"1747-0277","type":"print"},{"value":"1747-0285","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,4,7]]}}}