{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T23:35:24Z","timestamp":1792625724523,"version":"4.5.0"},"reference-count":14,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2015,7,20]],"date-time":"2015-07-20T00:00:00Z","timestamp":1437350400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Chem Biol Drug Des"],"published-print":{"date-parts":[[2015,12]]},"abstract":"<jats:p>Post\u2010traumatic arthritis is a secondary complication to severe joint trauma. With the disease progression, it may eventually lead to osteoarthritis in patients whose age is considerably younger than patients with traditional bone arthritis. The main objective of this study was to explore the feasibility of using lentiviral\u2010mediated RNA interference silencing of IL\u20101\u03b2 and TNF\u2010\u03b1 to treat post\u2010traumatic arthritis in rabbits. About 48 New Zealand rabbits underwent bilateral knee joint surgery to stimulate traumatic arthritis. They were then randomly divided into four groups of 12 rabbits each. The histopathology of the cartilage was observed, and the changes were assessed by Mankin scoring. ELISA was used to detect the expression of IL\u20101\u03b2 and TNF\u2010\u03b1 in the synovial fluid. (i) Compared with the control group, the transfection and co\u2010transfected groups displayed reduced cartilage damage and speed of degeneration. The co\u2010transfected group showed the greatest alleviation of symptoms. The Mankin score was statistically different (p\u00a0&lt;\u00a00.01). (ii) Compared with the control group, the expression of IL\u20101\u03b2 or TNF\u2010\u03b1 was reduced in the respective transfection groups (p\u00a0&lt;\u00a00.01 in both groups) and IL\u20101\u03b2 and TNF\u2010\u03b1 were reduced in the co\u2010transfected group (p\u00a0&lt;\u00a00.01). The co\u2010transfected group showed the lowest expression of the three experimental groups of both IL\u20101\u03b2 and TNF\u2010\u03b1 (p\u00a0&lt;\u00a00.01). Lentivirus\u2010mediated RNA interference can knock down the expression of IL\u20101\u03b2 and TNF\u2010\u03b1 in joint fluids and, in a synergistic effect when two siRNAs are co\u2010transfected, ease cartilage degeneration.<\/jats:p>","DOI":"10.1111\/cbdd.12611","type":"journal-article","created":{"date-parts":[[2015,6,24]],"date-time":"2015-06-24T11:07:31Z","timestamp":1435144051000},"page":"1466-1470","source":"Crossref","is-referenced-by-count":10,"title":["RNAi Silencing of IL\u20101\u03b2 and TNF\u2010\u03b1 in the Treatment of Post\u2010traumatic Arthritis in Rabbits"],"prefix":"10.1111","volume":"86","author":[{"given":"Qiang","family":"Tang","sequence":"first","affiliation":[{"name":"Department of Orthopedics The Second Affiliated Hospital of Nanchang University Nanchang 330006 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liang","family":"Hao","sequence":"additional","affiliation":[{"name":"Department of Orthopedics The Second Affiliated Hospital of Nanchang University Nanchang 330006 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanxiang","family":"Peng","sequence":"additional","affiliation":[{"name":"Department of Orthopedics The Second Affiliated Hospital of Nanchang University Nanchang 330006 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yating","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Orthopedics The Second Affiliated Hospital of Nanchang University Nanchang 330006 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kuo","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Orthopedics The Second Affiliated Hospital of Nanchang University Nanchang 330006 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng","family":"Cai","sequence":"additional","affiliation":[{"name":"Department of Orthopedics The Second Affiliated Hospital of Nanchang University Nanchang 330006 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunlong","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Orthopedics The Second Affiliated Hospital of Nanchang University Nanchang 330006 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi","family":"Liao","sequence":"additional","affiliation":[{"name":"Department of Orthopedics The Second Affiliated Hospital of Nanchang University Nanchang 330006 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,7,20]]},"reference":[{"key":"e_1_2_9_2_1","first-page":"517","article-title":"Post\u2010traumatic osteoarthritis: the role of stress induced chondrocyte damage","volume":"43","author":"Martin J.A.","year":"2006","journal-title":"Biorheology"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.blo.0000131638.81519.de"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.bor.0000151406.64393.00"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-133-5-200009050-00007"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/ar3046"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1006\/cyto.1998.0372"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-60761-588-0_1"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/35888"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1119(00)00224-9"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13277-011-0284-0"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/35078107"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1206858"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1261\/rna.2192803"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.orthres.2003.12.007"}],"container-title":["Chemical Biology &amp; 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