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In response to pyrazole compounds' antipyretic, analgesic, and anti\u2010inflammatory effects, this study sought to evaluate the analgesic, anti\u2010inflammatory, and vasorelaxant effects, as well as the mechanisms of action, of a new pyrazole derivative, 5\u2010[1\u2010(4\u2010fluorophenyl)\u20101<jats:italic>H<\/jats:italic>\u2010pyrazol\u20104\u2010yl]\u20102<jats:italic>H<\/jats:italic>\u2010tetrazole. During the acetic acid\u2010induced abdominal writhing test, treatments with 5\u2010[1\u2010(4\u2010fluorophenyl)\u20101<jats:italic>H<\/jats:italic>\u2010pyrazol\u20104\u2010yl]\u20102<jats:italic>H<\/jats:italic>\u2010tetrazole reduced abdominal writhing, while during the formalin test, 5\u2010[1\u2010(4\u2010fluorophenyl)\u20101<jats:italic>H<\/jats:italic>\u2010pyrazol\u20104\u2010yl]\u20102<jats:italic>H<\/jats:italic>\u2010tetrazole reduced licking times in response to both neurogenic pain and inflammatory pain, all without demonstrating any antinociceptive effects, as revealed during the tail flick test. 5\u2010[1\u2010(4\u2010fluorophenyl)\u20101<jats:italic>H<\/jats:italic>\u2010pyrazol\u20104\u2010yl]\u20102<jats:italic>H<\/jats:italic>\u2010tetrazole also reduced carrageenan\u2010induced paw edema and cell migration during the carrageenan\u2010induced pleurisy test. As demonstrated by the model of the isolated organ, 5\u2010[1\u2010(4\u2010fluorophenyl)\u20101<jats:italic>H<\/jats:italic>\u2010pyrazol\u20104\u2010yl]\u20102<jats:italic>H<\/jats:italic>\u2010tetrazole exhibits a vasorelaxant effect attenuated by N\u03c9\u2010nitro\u2010<jats:sc>l<\/jats:sc>\u2010arginine methyl ester, 1H\u2010[1,2,4]oxadiazolo[4,3\u2010alpha]quinoxalin\u20101\u2010one, tetraethylammonium or glibenclamide. 5\u2010[1\u2010(4\u2010fluorophenyl)\u20101<jats:italic>H<\/jats:italic>\u2010pyrazol\u20104\u2010yl]\u20102<jats:italic>H<\/jats:italic>\u2010tetrazole also blocked CaCl<jats:sub>2<\/jats:sub>\u2010induced contraction in a dose\u2010dependent manner. Suggesting a safe toxicity profile, 5\u2010[1\u2010(4\u2010fluorophenyl)\u20101<jats:italic>H<\/jats:italic>\u2010pyrazol\u20104\u2010yl]\u20102<jats:italic>H<\/jats:italic>\u2010tetrazole reduced the viability of 3T3 cells at higher concentrations and was orally tolerated, despite signs of toxicity in doses of 2000\u00a0mg\/kg. Lastly, the compounds' analgesic activity might be attributed to the involvement of the NO\/cGMP pathway and K<jats:sup>+<\/jats:sup>channels observed in the vasorelaxant effect.<\/jats:p>","DOI":"10.1111\/cbdd.12838","type":"journal-article","created":{"date-parts":[[2016,8,16]],"date-time":"2016-08-16T07:26:41Z","timestamp":1471332401000},"page":"124-135","source":"Crossref","is-referenced-by-count":20,"title":["New pyrazole derivative 5\u2010[1\u2010(4\u2010fluorophenyl)\u20101H\u2010pyrazol\u20104\u2010yl]\u20102H\u2010tetrazole: synthesis and assessment of some biological activities"],"prefix":"10.1111","volume":"89","author":[{"given":"Lanussy Porfiro","family":"de Oliveira","sequence":"first","affiliation":[{"name":"Laboratory of Pharmacology of Natural and Synthetic Products Department of Pharmacology Institute of Biological Sciences Federal University of Goi\u00e1s Goi\u00e2nia GO Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daiany Priscilla 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