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This new compound was subjected to pharmacological screening referred to as Irwin test, prior to sodium pentobarbital\u2010induced sleep, open\u2010field and wire tests. The anxiolytic\u2010like effect of this compound was evaluated using elevated plus maze and light\u2013dark box tests. In addition, the mnemonic activity was evaluated through step\u2010down test. In sodium pentobarbital\u2010induced sleep test, <jats:styled-content style=\"fixed-case\">LQFM<\/jats:styled-content>032 decreased latency and increased duration of sleep. In the open\u2010field test, <jats:styled-content style=\"fixed-case\">LQFM<\/jats:styled-content>032 altered behavioral parameter, that suggested anxiolytic\u2010like activity, as increased in crossings and time spent at the center of open field. In the plus maze test and light\u2013dark box test, the <jats:styled-content style=\"fixed-case\">LQFM<\/jats:styled-content>032 showed anxiolytic\u2010like activity, increased entries and time spent on open arms, and increased in number of transitions and time spent on light area, respectively. Those effects was antagonized by flumazenil but not with 1\u2010(2\u2010Methoxyphenyl)\u20104\u2010(4\u2010phthalimidobutyl)piperazine (<jats:styled-content style=\"fixed-case\">NAN<\/jats:styled-content>\u2010190). The <jats:styled-content style=\"fixed-case\">LQFM<\/jats:styled-content>032 did not alter mnemonic activity. Moreover, the anxiolytic\u2010like activity of <jats:styled-content style=\"fixed-case\">LQFM<\/jats:styled-content>032 was antagonized by mecamylamine. In summary, <jats:styled-content style=\"fixed-case\">LQFM<\/jats:styled-content>032 showed benzodiazepine and nicotinic pathways mediated anxiolytic\u2010like activity without altering the mnemonic activity.<\/jats:p>","DOI":"10.1111\/cbdd.12961","type":"journal-article","created":{"date-parts":[[2017,2,5]],"date-time":"2017-02-05T01:45:09Z","timestamp":1486259109000},"page":"432-442","source":"Crossref","is-referenced-by-count":13,"title":["Anxiolytic\u2010like effect of 2\u2010(4\u2010((1\u2010phenyl\u20101<i>H<\/i>\u2010pyrazol\u20104\u2010yl)methyl)piperazin\u20101\u2010yl)ethan\u20101\u2010ol is mediated through the benzodiazepine and nicotinic pathways"],"prefix":"10.1111","volume":"90","author":[{"given":"Adriane F.","family":"Brito","sequence":"first","affiliation":[{"name":"Department of Pharmacology ICB Federal University of Goi\u00e1s  Goi\u00e2nia GO Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James O.","family":"Fajemiroye","sequence":"additional","affiliation":[{"name":"Department of Pharmacology ICB Federal University of Goi\u00e1s  Goi\u00e2nia GO Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiasmin F. 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