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Due to severe health consequences and treatment failure, new therapeutic alternatives are crucial. Phloroglucinols from southern Brazilian <jats:italic>Hypericum<\/jats:italic> species demonstrated anti\u2010<jats:italic>T.\u00a0vaginalis<\/jats:italic> and anti\u2010<jats:italic>Leishmania amazonensis<\/jats:italic> activities. The modulation of biochemical pathways involved in the control of inflammatory response by ectonucleotidases, NTPDase, and ecto\u20105\u2032\u2010nucleotidase represents new targets for combating protozoa. This study investigated the activity of phloroglucinol derivatives of <jats:italic>Hypericum<\/jats:italic> species from southern Brazil against <jats:italic>T.\u00a0vaginalis<\/jats:italic> as well as its ability on modulating parasite ectonucleotidases and, consequently, immune parameters through ATP and adenosine effects. Phloroglucinol derivatives screening revealed activity for isoaustrobrasilol B (IC<jats:sub>50<\/jats:sub> 38\u00a0\u03bc<jats:sc>m<\/jats:sc>) with no hemolytic activity. Although the most active compound induced cytotoxicity against a mammalian cell lineage, the in vivo model evidenced absence of toxicity. Isoaustrobrasilol B significantly inhibited NTPDase and ecto\u20105\u2032\u2010nucleotidase activities, and the immune modulation attributed to extracellular nucleotide accumulation was evaluated. The production of ROS and IL\u20106 by <jats:italic>T.\u00a0vaginalis\u2010<\/jats:italic>stimulated neutrophils was not affected by the treatment. Conversely, IL\u20108 levels were significantly enhanced. The associative mechanism of trophozoites death and ectonucleotidases modulation by isoaustrobrasilol B may increase the susceptibility of <jats:italic>T.\u00a0vaginalis<\/jats:italic> to host innate immune cell like neutrophils consequently, contributing to parasite clearance.<\/jats:p>","DOI":"10.1111\/cbdd.13002","type":"journal-article","created":{"date-parts":[[2017,4,8]],"date-time":"2017-04-08T08:53:22Z","timestamp":1491641602000},"page":"811-819","source":"Crossref","is-referenced-by-count":16,"title":["The anti\u2010<i>Trichomonas vaginalis<\/i> phloroglucinol derivative isoaustrobrasilol B modulates extracellular nucleotide hydrolysis"],"prefix":"10.1111","volume":"90","author":[{"given":"Camila Braz","family":"Menezes","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Pesquisa em Parasitologia Faculdade de Farm\u00e1cia Universidade Federal do Rio Grande do Sul  Porto Alegre RS Brazil"},{"name":"Programa de P\u00f3s\u2010Gradua\u00e7\u00e3o em Ci\u00eancias Farmac\u00eauticas Faculdade de Farm\u00e1cia Universidade Federal do Rio Grande do Sul  Porto Alegre RS Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Graziela Vargas","family":"Rigo","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Pesquisa em Parasitologia Faculdade de Farm\u00e1cia Universidade Federal do Rio Grande do Sul  Porto Alegre RS Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henrique","family":"Bridi","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s\u2010Gradua\u00e7\u00e3o em Ci\u00eancias Farmac\u00eauticas Faculdade de Farm\u00e1cia Universidade Federal do Rio Grande do Sul  Porto Alegre RS Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danielle da Silva","family":"Trentin","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s\u2010Gradua\u00e7\u00e3o em Ci\u00eancias Farmac\u00eauticas Faculdade de Farm\u00e1cia Universidade Federal do Rio Grande do Sul  Porto Alegre RS Brazil"},{"name":"Departamento de Ci\u00eancias B\u00e1sicas da Sa\u00fade Universidade Federal de Ci\u00eancias da Sa\u00fade de Porto Alegre  Porto Alegre RS Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandre Jos\u00e9","family":"Macedo","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s\u2010Gradua\u00e7\u00e3o em Ci\u00eancias Farmac\u00eauticas Faculdade de Farm\u00e1cia Universidade Federal do Rio Grande do Sul  Porto Alegre RS Brazil"},{"name":"Faculdade de Farm\u00e1cia e Centro de Biotecnologia Universidade Federal do Rio Grande do Sul  Porto Alegre RS Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gilsane Lino","family":"von Poser","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s\u2010Gradua\u00e7\u00e3o em Ci\u00eancias Farmac\u00eauticas Faculdade de Farm\u00e1cia Universidade Federal do Rio Grande do Sul  Porto Alegre RS Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8334-5105","authenticated-orcid":false,"given":"Tiana","family":"Tasca","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Pesquisa em Parasitologia Faculdade de Farm\u00e1cia Universidade Federal do Rio Grande do Sul  Porto Alegre RS Brazil"},{"name":"Programa de P\u00f3s\u2010Gradua\u00e7\u00e3o em Ci\u00eancias Farmac\u00eauticas Faculdade de Farm\u00e1cia Universidade Federal do Rio Grande do Sul  Porto Alegre RS Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,5,14]]},"reference":[{"key":"e_1_2_7_2_1","volume-title":"Global incidence and prevalence of selected curable sexually transmitted infections \u2013 2008","author":"World Health Organization","year":"2012"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.13-0723"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1136\/sextrans-2013-051075"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1080\/028418600431003"},{"key":"e_1_2_7_6_1","doi-asserted-by":"crossref","first-page":"682","DOI":"10.1093\/ije\/23.4.682","volume":"23","author":"Zhang Z. 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