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The \u03b16\u03b22* nAChR is an important subtype, which is closely associated with nicotine addiction and movement disorders etc. \u03b1\u2010conotoxin TxIB with 16\u2010amino acid residues specifically targets \u03b16\u03b22* nAChR with no obvious effect on other nAChR subtypes. However, chemical synthesis of TxIB is expensive, and the quantity of native TxIB extracted from cone snail is limited. In the present study, we attempted to obtain TxIB using biological method based on the recombinant expression in <jats:italic>Escherichia coli<\/jats:italic> (<jats:italic>E.\u00a0coli<\/jats:italic>). The synthetic gene encoding mature peptide of TxIB was inserted in pET\u201031b(+) vector and transformed into <jats:italic>E.\u00a0coli<\/jats:italic> strain BLR(DE3)pLysS for expression. The recombinant fusion protein KSI\u2010TxIB\u2010His<jats:sub>6<\/jats:sub> (KSI, ketosteroid isomerase) was expressed successfully as inclusion body in <jats:italic>E.\u00a0coli<\/jats:italic>, which was purified by Ni\u2010NTA affinity chromatography column and cleaved by cyanogen bromide (CNBr) to release recombinant \u03b1\u2010conotoxin TxIB (rTxIB). Then, rTxIB was purified by reverse\u2010phase high\u2010performance liquid chromatography (RP\u2010HPLC) and was identified by electrospray ionization mass spectrometry (ESI\u2010MS). Pharmacological activity of rTxIB was assessed by electrophysiological approaches. The results indicated that it preserved about 50% of potency, but, was even more important, had the same selectivity as the natural conotoxin which may provide an alternative method for quantity production of small peptides with low cost on the premise of not changing their potency.<\/jats:p>","DOI":"10.1111\/cbdd.13104","type":"journal-article","created":{"date-parts":[[2017,9,11]],"date-time":"2017-09-11T09:22:44Z","timestamp":1505121764000},"page":"349-358","source":"Crossref","is-referenced-by-count":12,"title":["Expression in <i>Escherichia coli<\/i> of fusion protein comprising \u03b1\u2010conotoxin Tx<scp>IB<\/scp> and preservation of selectivity to nicotinic acetylcholine receptors in the purified product"],"prefix":"10.1111","volume":"91","author":[{"given":"Jinpeng","family":"Yu","sequence":"first","affiliation":[{"name":"Key Laboratory of Tropical Biological Resources Ministry of Education Key Laboratory for Marine Drugs of Haikou Hainan University  Haikou China"},{"name":"College of Agriculture Hainan University  Haikou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaopeng","family":"Zhu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Tropical Biological Resources Ministry of Education Key Laboratory for Marine Drugs of Haikou Hainan University  Haikou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Yang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Tropical Biological Resources Ministry of Education Key Laboratory for Marine Drugs of Haikou Hainan University  Haikou China"},{"name":"College of Agriculture Hainan University  Haikou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sulan","family":"Luo","sequence":"additional","affiliation":[{"name":"Key Laboratory of Tropical Biological Resources Ministry of Education Key Laboratory for Marine Drugs of Haikou Hainan University  Haikou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6813-4521","authenticated-orcid":false,"given":"Dongting","family":"Zhangsun","sequence":"additional","affiliation":[{"name":"Key Laboratory of Tropical Biological Resources Ministry of Education Key Laboratory for Marine Drugs of Haikou Hainan University  Haikou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,9,25]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00015.2008"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuropharm.2008.07.041"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.11-195883"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.5095-09.2010"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2011.06.001"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.10-166272"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2006.07.023"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.peptides.2009.03.019"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1501334112"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112.427898"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coph.2005.01.013"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1124\/mol.110.066159"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.3390\/md14010011"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2013.06.012"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-004-1559-9"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pep.2009.02.002"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12033-013-9667-3"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.13-244103"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.3390\/md11093537"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-540-30880-5_4"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.260410103"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.2174\/138161211798999384"},{"key":"e_1_2_8_24_1","first-page":"574","volume":"52","author":"Zhangsun D.","year":"2017","journal-title":"Chin. 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