{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T11:41:41Z","timestamp":1778758901959,"version":"3.51.4"},"reference-count":41,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2018,6,26]],"date-time":"2018-06-26T00:00:00Z","timestamp":1529971200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100008725","name":"Agencia Nacional de Investigaci\u00f3n e Innovaci\u00f3n","doi-asserted-by":"publisher","award":["POS_FMV_2015_1_1005183"],"award-info":[{"award-number":["POS_FMV_2015_1_1005183"]}],"id":[{"id":"10.13039\/100008725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006049","name":"Comisi\u00f3n Sectorial de Investigaci\u00f3n Cient\u00edfica","doi-asserted-by":"publisher","award":["INI_2015_362"],"award-info":[{"award-number":["INI_2015_362"]}],"id":[{"id":"10.13039\/501100006049","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chem Biol Drug Des"],"published-print":{"date-parts":[[2018,9]]},"abstract":"<jats:p>Chagas disease is an endemic illness in Latin America caused by the parasite <jats:italic>Trypanosoma cruzi<\/jats:italic>. Current chemotherapies are old and inadequate, and the emergence of drug\u2010resistant strains underscores the need of new drugs. Platinum\u2010based complexes have been shown to be a promising approach against parasitic diseases. In this work, the effect of 1,1\u2032\u2010bis(diphenylphosphino)ferrocene pyridine\u20102\u2010thiolate\u20101\u2010oxide Pt(<jats:styled-content style=\"fixed-case\">II<\/jats:styled-content>) hexafluorophosphate, Pt\u2010dppf\u2010mpo, was studied on <jats:italic>T.\u00a0cruzi<\/jats:italic>. A promising antitrypanosomal activity was determined for the <jats:styled-content style=\"fixed-case\">CL<\/jats:styled-content> Brener strain with a low cytotoxicity determined using in vitro\u2010cultured mammal cells. The compound uptake in parasites treated with concentrations of 1\u00d7 and 10\u00d7 the <jats:styled-content style=\"fixed-case\">IC<\/jats:styled-content><jats:sub>50<\/jats:sub> value reached ~75% and 19%, respectively. Pt\u2010dppf\u2010mpo induced necrosis after 24\u00a0hr of parasite incubation. This event was preceded by depolarization of mitochondrial membrane potential. Cell vitality assays showed high esterase activity in treated parasites. However, despite this increase in metabolic activity, treated epimastigotes showed rounded morphology and loss of flagellum with a reduction in mobility as compound concentration and\/or time of incubation was increased. At last, we demonstrate that Pt\u2010dppf\u2010mpo incubation also affects the trypomastigote infection process as well as the infection persistence evaluated as the number of amastigotes per cell in a dose\u2010dependent manner.<\/jats:p>","DOI":"10.1111\/cbdd.13332","type":"journal-article","created":{"date-parts":[[2018,5,10]],"date-time":"2018-05-10T03:49:51Z","timestamp":1525924191000},"page":"1657-1669","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["<i>Trypanosoma cruzi<\/i> biochemical changes and cell death induced by an organometallic platinum\u2010based compound"],"prefix":"10.1111","volume":"92","author":[{"given":"M. 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