{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T16:09:18Z","timestamp":1787069358577,"version":"build-2736575974"},"reference-count":30,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2018,6,19]],"date-time":"2018-06-19T00:00:00Z","timestamp":1529366400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R15AI128585"],"award-info":[{"award-number":["R15AI128585"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chem Biol Drug Des"],"published-print":{"date-parts":[[2018,10]]},"abstract":"<jats:p>\n                    <jats:italic>Trichomonas vaginalis<\/jats:italic>\n                    infects approximately 300\u00a0million people worldwide annually. Infected individuals have a higher susceptibility to more serious conditions such as cervical and prostate cancer. The parasite has developed increasing resistance to current drug therapies, with an estimated 5% of clinical cases resulting from resistant strains, creating the need for new therapeutic strategies with novel mechanisms of action. Nucleoside salvage pathway enzymes represent novel drug targets as these pathways are essential for the parasite's survival. The guanosine\/adenosine\/cytidine nucleoside hydrolase (\n                    <jats:styled-content style=\"fixed-case\">GACNH<\/jats:styled-content>\n                    ) may be particularly important as its expression is upregulated under glucose\u2010limiting conditions mimicking those that occur during infection establishment.\n                    <jats:styled-content style=\"fixed-case\">GACNH<\/jats:styled-content>\n                    was screened against the\n                    <jats:styled-content style=\"fixed-case\">NIH<\/jats:styled-content>\n                    Clinical Collection to explore its druggability. Seven compounds were identified with\n                    <jats:styled-content style=\"fixed-case\">IC<\/jats:styled-content>\n                    <jats:sub>50<\/jats:sub>\n                    values &lt;20\u00a0\u03bcM. Extensive overlap was found between inhibitors of\n                    <jats:styled-content style=\"fixed-case\">GACNH<\/jats:styled-content>\n                    and the adenosine\/guanosine nucleoside hydrolase (\n                    <jats:styled-content style=\"fixed-case\">AGNH<\/jats:styled-content>\n                    ), but no overlap was found with inhibitors of the uridine nucleoside hydrolase. The guanosine analog ribavirin was the only compound found to be specific for\n                    <jats:styled-content style=\"fixed-case\">GACNH<\/jats:styled-content>\n                    . Compounds that inhibit both\n                    <jats:styled-content style=\"fixed-case\">AGNH<\/jats:styled-content>\n                    and\n                    <jats:styled-content style=\"fixed-case\">GACNH<\/jats:styled-content>\n                    purine salvage pathway enzymes may prove critical given the role that\n                    <jats:styled-content style=\"fixed-case\">GACNH<\/jats:styled-content>\n                    appears to play in the early stages of infection.\n                  <\/jats:p>","DOI":"10.1111\/cbdd.13341","type":"journal-article","created":{"date-parts":[[2018,5,29]],"date-time":"2018-05-29T02:16:12Z","timestamp":1527560172000},"page":"1736-1742","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Druggability of the guanosine\/adenosine\/cytidine nucleoside hydrolase from\n                    <i>Trichomonas vaginalis<\/i>"],"prefix":"10.1111","volume":"92","author":[{"given":"Rayyan","family":"Alam","sequence":"first","affiliation":[{"name":"Department of Chemistry Adelphi University  Garden City New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Allen T.","family":"Barbarovich","sequence":"additional","affiliation":[{"name":"Department of Chemistry Adelphi University  Garden City New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wagma","family":"Caravan","sequence":"additional","affiliation":[{"name":"Department of Chemistry Adelphi University  Garden City New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mirna","family":"Ismail","sequence":"additional","affiliation":[{"name":"Department of Chemistry Adelphi University  Garden City New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela","family":"Barskaya","sequence":"additional","affiliation":[{"name":"Department of Chemistry Adelphi University  Garden City New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David W.","family":"Parkin","sequence":"additional","affiliation":[{"name":"Department of Chemistry Adelphi University  Garden City New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8520-9588","authenticated-orcid":false,"given":"Brian J.","family":"Stockman","sequence":"additional","affiliation":[{"name":"Department of Chemistry Adelphi University  Garden City New York"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,6,19]]},"reference":[{"key":"e_1_2_4_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-015-1055-0"},{"key":"e_1_2_4_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/gbe\/evx110"},{"key":"e_1_2_4_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00109-16"},{"key":"e_1_2_4_5_1","unstructured":"http:\/\/www.cdc.gov\/std\/trichomonas\/stats.htm(accessed: March 2018)."},{"key":"e_1_2_4_6_1","doi-asserted-by":"publisher","DOI":"10.1586\/eri.11.159"},{"key":"e_1_2_4_7_1","doi-asserted-by":"publisher","DOI":"10.1097\/QCO.0000000000000128"},{"key":"e_1_2_4_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/djp306"},{"key":"e_1_2_4_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00430-011-0205-2"},{"key":"e_1_2_4_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1321884111"},{"key":"e_1_2_4_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.17.4.783-793.2004"},{"key":"e_1_2_4_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(82)80026-4"},{"key":"e_1_2_4_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(84)80910-2"},{"key":"e_1_2_4_14_1","first-page":"54","volume":"2010","author":"Berg M.","year":"1900","journal-title":"Antimicrob. 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