{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T23:41:48Z","timestamp":1789429308967,"version":"build-2803163510"},"reference-count":31,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2019,3,13]],"date-time":"2019-03-13T00:00:00Z","timestamp":1552435200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100010679","name":"Pasteur Institute of Iran","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100010679","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chem Biol Drug Des"],"published-print":{"date-parts":[[2019,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The antimicrobial activities of natural products have attracted much attention due to the increasing incidence of pathogens that have become resistant to drugs. Thus, it has been attempted to promisingly manage infectious diseases via a new group of therapeutic agents called antimicrobial peptides. In this study, a novel antifungal peptide, MCh\u2010AMP1<jats:italic>,<\/jats:italic> was purified by reverse phase HPLC and sequenced by de novo sequencing and Edman degradation. The antifungal activity, safety, thermal, and pH stability of MCh\u2010AMP1 were determined. This peptide demonstrated an antifungal activity against the tested <jats:italic>Candida<\/jats:italic> and <jats:italic>Aspergillus<\/jats:italic> species with MIC values in the range of 3.33\u20136.66\u00a0\u03bcM and 6.66\u201313.32\u00a0\u03bcM, respectively. Further, physicochemical properties and molecular modeling of MCh\u2010AMP1 were evaluated. MCh\u2010AMP1 demonstrated 3.65% hemolytic activity at the concentration of 13.32\u00a0\u03bcM on human red blood cells and 10% toxicity after 48\u00a0hr at the same concentration on HEK293 cell lines. The antifungal activity of MCh\u2010AMP1 against <jats:italic>Candida albicans<\/jats:italic> was stable at a temperature range of 30\u201350\u00b0C and at the pH level of 7\u201311. The present study indicates that MCh\u2010AMP1 may be considered as a new antifungal agent with therapeutic potential against major human pathogenic fungi.<\/jats:p>","DOI":"10.1111\/cbdd.13500","type":"journal-article","created":{"date-parts":[[2019,2,18]],"date-time":"2019-02-18T08:04:31Z","timestamp":1550477071000},"page":"949-959","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Isolation, functional characterization, and biological properties of MCh\u2010AMP1, a novel antifungal peptide from <i>Matricaria chamomilla<\/i> L."],"prefix":"10.1111","volume":"93","author":[{"given":"Sima Sadat","family":"Seyedjavadi","sequence":"first","affiliation":[{"name":"Department of Mycology Pasteur Institute of Iran  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soghra","family":"Khani","sequence":"additional","affiliation":[{"name":"Department of Mycology Pasteur Institute of Iran  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1501-2560","authenticated-orcid":false,"given":"Hadi","family":"Zare\u2010Zardini","sequence":"additional","affiliation":[{"name":"Hematology and Oncology Research Center Shahid Sadoughi University of Medical Sciences  Yazd Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raheleh","family":"Halabian","sequence":"additional","affiliation":[{"name":"Applied Microbiology Research Center Systems Biology and Poisonings Institute Baqiyatallah University of Medical Sciences  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mehdi","family":"Goudarzi","sequence":"additional","affiliation":[{"name":"Department of Microbiology School of Medicine Shahid Beheshti University of Medical Sciences  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shohreh","family":"Khatami","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Pasteur Institute of Iran  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abbas Ali","family":"Imani Fooladi","sequence":"additional","affiliation":[{"name":"Applied Microbiology Research Center Systems Biology and Poisonings Institute Baqiyatallah University of Medical Sciences  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jafar","family":"Amani","sequence":"additional","affiliation":[{"name":"Applied Microbiology Research Center Systems Biology and Poisonings Institute Baqiyatallah University of Medical Sciences  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8217-399X","authenticated-orcid":false,"given":"Mehdi","family":"Razzaghi\u2010Abyaneh","sequence":"additional","affiliation":[{"name":"Department of Mycology Pasteur Institute of Iran  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,3,13]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/psc.1409"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2796.2003.01228.x"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00132a023"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/abbs\/gms003"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00925-06"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.4155\/fmc-2016-0035"},{"key":"e_1_2_8_8_1","unstructured":"Gaiser R. 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