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This need provides an opportunity for the development of antimicrobial peptides (AMPs). To develop new AMPs with effective and safe therapeutic effects, two PMAP\u201037 analogs called PMAP\u201037(R13\u2010I) and PMAP\u201037(K20\/27\u2010I) were designed to increase hydrophobicity. Antimicrobial susceptibility testing and animal infection models were used to assess their antibacterial activity. The results showed that the minimal inhibitory concentrations of PMAP\u201037(R13\u2010I) were lower than those of PMAP\u201037 for two gram\u2010negative strains. Compared with PMAP\u201037, PMAP\u201037(K20\/27\u2010I) not only inhibited the growth of most bacterial strains, but also exhibited antibacterial activity against <jats:italic>Shigella flexneri<\/jats:italic> CICC21534. In addition, PMAP\u201037(K20\/27\u2010I) exhibited pH and thermal stability. PMAP\u201037(R13\u2010I) had a therapeutic effect only in mice infected with <jats:italic>Salmonella typhimurium<\/jats:italic> SL1344. However, PMAP\u201037(K20\/27\u2010I) exhibited the therapeutic effects, whether in the clinical symptoms, the tissue lesions, or the tissue bacterial loads and the survival rates in mice infected with <jats:italic>Staphylococcus aureus<\/jats:italic> ATCC25923 or <jats:italic>S.\u00a0typhimurium<\/jats:italic> SL1344. Therefore, PMAP\u201037(K20\/27\u2010I) can be used as a substitute for antibiotics against infection with bacterial strains.<\/jats:p>","DOI":"10.1111\/cbdd.13601","type":"journal-article","created":{"date-parts":[[2019,8,22]],"date-time":"2019-08-22T20:44:27Z","timestamp":1566506667000},"page":"1986-1999","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Enhancing the antibacterial activity of PMAP\u201037 by increasing its hydrophobicity"],"prefix":"10.1111","volume":"94","author":[{"given":"Jiangfei","family":"Zhou","sequence":"first","affiliation":[{"name":"College of Animal Science and Technology Henan University of Science and Technology Luoyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yongqing","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology Henan University of Science and Technology Luoyang 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