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However, there is scarce knowledge regarding Trp residues arrangement at the head group level. Thus, a set of \u03b1\u2010helical AMPs containing different Trp residue arrangements at the N\u2010\/C\u2010terminal of sequence were designed to increase the strategy database and analyze their biological activities. The arrangement of the N\u2010terminal Trp residue significantly improved the bacteriostatic activity of the peptides, but the C\u2010terminal Trp residue arrangement reduced the biocompatibility of them. WL and LW were effective against Gram\u2010negative microbes and had high selectivity for bacteria as compared to human erythrocytes and mammalian cells. They both maintained a relatively desirable activity in the presence of physiological salts and serum. It is observed through electron microscope, flow cytometry and fluorescence spectroscopy that target peptides can penetrate bacterial cell membrane and kill it by damaging cell membrane integrity. Collectively, we determined the structure\u2013activity relationship of Trp residue distributions in a symmetric sequence structure and filled the gap in knowledge related to Trp arrangements at the head group level. The obtained results will be helpful in designing of artificial peptide\u2010based antimicrobials.<\/jats:p>","DOI":"10.1111\/cbdd.13608","type":"journal-article","created":{"date-parts":[[2019,8,23]],"date-time":"2019-08-23T19:03:38Z","timestamp":1566587018000},"page":"2051-2063","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Effect of terminal arrangement of tryptophan on biological activity of symmetric \u03b1\u2010helix\u2010forming peptides"],"prefix":"10.1111","volume":"94","author":[{"given":"Changxuan","family":"Shao","sequence":"first","affiliation":[{"name":"Institute of Animal Nutrition Northeast Agricultural University Harbin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weizhong","family":"Li","sequence":"additional","affiliation":[{"name":"Institute of Animal Nutrition Northeast Agricultural University Harbin China"},{"name":"College of Biological and Agricultural 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