{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T06:15:59Z","timestamp":1785392159547,"version":"3.55.0"},"reference-count":26,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2021,1,23]],"date-time":"2021-01-23T00:00:00Z","timestamp":1611360000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chem Biol Drug Des"],"published-print":{"date-parts":[[2021,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>FIKK\u20109.1 is essential for parasite survival, but its structural and biochemical characterization will enable us to understand its role in the parasite life cycle. The recombinant FIKK9.1 kinase is monomeric with a native molecular weight of 60\u00a0\u00b1\u00a01.6\u00a0kDa. Structural characterization of FIKK9.1 kinase reveals that it consists of two domains: N\u2010terminal FHA like domain and C\u2010terminal kinase domain. The C\u2010terminal domain has a well\u2010defined pocket, but it displayed RMSD deviation of 1.38\u20133.2\u00a0\u00c5 from host kinases. ITC analysis indicates that ATP binds to the protein with a <jats:italic>K<\/jats:italic><jats:sub>d<\/jats:sub> of 45.6\u00a0\u00b1\u00a02.4\u00a0\u00b5M. Mutational studies confirm the role of Val\u2010244, Met\u2010245, Lys\u2010320, 324, and Glu\u2010366 for ATP binding. Co\u2010localization studies revealed FIKK9.1 in the parasite cytosol with a component trafficked to the apicoplast and also to IRBC. FIKK9.1 has 23 pockets to serve as potential docking sites for substrates. Correlation analysis of peptides from the combinatorial library concluded that peptide P277 (MFDFHYTLGPMWGTL) was fitting nicely into the binding pocket. The peptide P277 picked up candidates from parasite and key players from RBC cytoskeleton. Interestingly, FIKK9.1 is phosphorylating spectrin, ankyrin, and band\u20103 from RBC cytoskeleton. Our study highlights the structural and biochemical features of FIKK9.1 to exploit it as a drug target.<\/jats:p>","DOI":"10.1111\/cbdd.13821","type":"journal-article","created":{"date-parts":[[2021,1,24]],"date-time":"2021-01-24T02:23:15Z","timestamp":1611454995000},"page":"962-977","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["<i>Plasmodium falciparum<\/i> FIKK9.1 is a monomeric serine\u2013threonine protein kinase with features to exploit as a drug target"],"prefix":"10.1111","volume":"97","author":[{"given":"Anil Kumar","family":"D.","sequence":"first","affiliation":[{"name":"Malaria Research Group Department of Biosciences and Bioengineering Indian Institute of Technology\u2010Guwahati Guwahati Assam India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deepti","family":"Shrivastava","sequence":"additional","affiliation":[{"name":"Molecular and Structural Biology Division CSIR\u2010Central Drug Research Institute Lucknow Uttar Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amogh A.","family":"Sahasrabuddhe","sequence":"additional","affiliation":[{"name":"Molecular and Structural Biology Division CSIR\u2010Central Drug Research Institute Lucknow Uttar Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saman","family":"Habib","sequence":"additional","affiliation":[{"name":"Molecular and Structural Biology Division CSIR\u2010Central Drug Research Institute Lucknow Uttar Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vishal","family":"Trivedi","sequence":"additional","affiliation":[{"name":"Malaria Research Group Department of Biosciences and Bioengineering Indian Institute of Technology\u2010Guwahati Guwahati Assam India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,1,23]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.4381"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molbiopara.2013.08.003"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jproteome.7b00025"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(01)03294-X"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/1475-2875-8-99"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature01097"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(05)66420-3"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep39285"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsb.2007.09.005"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2014.01.003"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1021\/cr040409x"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tips.2008.02.005"},{"issue":"51","key":"e_1_2_8_14_1","first-page":"re12","article-title":"Structure and function of the phosphothreonine\u2010specific FHA domain","volume":"1","author":"Mahajan A.","year":"2008","journal-title":"Science Signalling"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.idc.2012.03.010"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2006.05521.x"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0011747"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr400394g"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkn483"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-6-30"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molbiopara.2004.05.009"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2013.06.001"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2008.12.048"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-397918-6.00001-X"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-5-79"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1186\/1475-2875-8-105"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.2147\/IDR.S123887"}],"container-title":["Chemical Biology &amp; 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