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Use of laboratory animals such as mice, rat, and rabbits was considered as gold standard in herbal toxicity assessments. However, in the last few decades, the ethical consideration of using higher vertebrates for toxicity testing has become more controversial. As a possible alternative model involving lower vertebrates such as zebra fish were introduced. Hence in the present study, swertiamain compound isolated from <jats:italic>E. axillare<\/jats:italic> was assessed for it antimicrobial activity in zebra fish larvae againt <jats:italic>S. typhi<\/jats:italic>. The cumulative mortality rate and bacterial localization in zebra fish larvae were studied. Biochemical markers assays were performed to find the preventive role of the compound during the typhoid infection. The results showed that zebra fish can be successfully used as a model to study typhoid infection and the anti\u2010bacterial compound swertiamarin used in this study clears the bacterial load and pathogenic symptoms to a great extent.<\/jats:p>","DOI":"10.1111\/cbdd.14146","type":"journal-article","created":{"date-parts":[[2022,9,30]],"date-time":"2022-09-30T10:40:37Z","timestamp":1664534437000},"page":"1033-1041","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Effect of novel therapeutic medicine swertiamarin from <i>Enicostema axillare<\/i> in zebrafish infected with <i>Salmonella typhi<\/i>"],"prefix":"10.1111","volume":"100","author":[{"given":"Sasidharan","family":"Perumal","sequence":"first","affiliation":[{"name":"Department of Plant Biotechnology, School of Biotechnology Madurai Kamaraj University  Madurai India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"MadhanaVigneshwari Gopal","family":"Samy","sequence":"additional","affiliation":[{"name":"Department of Zoology GTN Arts College  Dindigul India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dharaneedharan","family":"Subramanian","sequence":"additional","affiliation":[{"name":"Department of Zoology GTN Arts College  Dindigul India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,10,21]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1098\/rspa.2020.0354"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V98.3.643"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1139\/y2012-055"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.333.7558.78"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12916\u2010019\u20101443\u20101"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1089\/zeb.2008.0552"},{"key":"e_1_2_10_8_1","first-page":"1473","volume-title":"Feigin and Cherry's Textbook of Pediatric Infectious Diseases","author":"Cleary T.","year":"2005"},{"key":"e_1_2_10_9_1","first-page":"346","article-title":"The global burden of enteric fever","volume":"82","author":"Crump J. 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