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Our proposal seeks to contribute to developing a new agent that meets the needs of diagnosis and follow\u2010up of fungal and bacterial infections, focused on the design of a radiotracer with the potential for recognition of hidden infection foci. Defensins are plant antimicrobial peptides that not only show activity against plant pathogens but also against human ones. A short analogue of EcgDf1 defensin, EcgDf21d (NH<jats:sub>2<\/jats:sub>\u2010ERFTGGHCRGFRRRCFCTKHC\u2010COOH), was labelled through the formation of a <jats:sup>99m<\/jats:sup>Tc\u2010HYNIC complex which was assessed for physicochemical and biological behaviour both in vitro and in vivo. The [<jats:sup>99m<\/jats:sup>Tc]Tc\u2010HYNIC\u2010EcgDf21 labelling procedure rendered a single product with remarkably high RCP and stability in the labelling milieu. The Log <jats:italic>p<\/jats:italic> value indicated that [<jats:sup>99m<\/jats:sup>Tc]Tc\u2010HYNIC\u2010EcgDf21 has a hydrophilic behaviour, confirmed by the biodistribution profiles. The optimal uptake value was obtained for <jats:italic>Candida albicans<\/jats:italic> infection model reaching a lesion\/muscle ratio of 3, this correlates with in vitro binding studies, and the lesion can be definitely observed in the scintigraphic images.<\/jats:p>","DOI":"10.1111\/cbdd.14149","type":"journal-article","created":{"date-parts":[[2022,9,23]],"date-time":"2022-09-23T03:48:10Z","timestamp":1663904890000},"page":"593-604","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["[<scp><sup>99m<\/sup>Tc<\/scp>]<scp>Tc\u2010HYNIC<\/scp>\u2010<scp>EcgDf21<\/scp>: A defensin short analogue with potential application in infection foci imaging"],"prefix":"10.1111","volume":"101","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4566-0814","authenticated-orcid":false,"given":"Mariella","family":"Ter\u00e1n","sequence":"first","affiliation":[{"name":"\u00c1rea de Radioqu\u00edmica, Departamento Estrella Campos, Facultad de Qu\u00edmica Universidad de la Rep\u00fablica (Udelar)  Montevideo Uruguay"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jessica","family":"Osorio","sequence":"additional","affiliation":[{"name":"\u00c1rea de Radioqu\u00edmica, Departamento Estrella Campos, Facultad de Qu\u00edmica Universidad de la Rep\u00fablica (Udelar)  Montevideo Uruguay"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elena","family":"Cardoso","sequence":"additional","affiliation":[{"name":"\u00c1rea de Radioqu\u00edmica, Departamento Estrella Campos, Facultad de Qu\u00edmica Universidad de la Rep\u00fablica (Udelar)  Montevideo Uruguay"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emilia","family":"Tejer\u00eda","sequence":"additional","affiliation":[{"name":"\u00c1rea de Radioqu\u00edmica, Departamento Estrella Campos, Facultad de Qu\u00edmica Universidad de la Rep\u00fablica (Udelar)  Montevideo Uruguay"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrea","family":"Paolino","sequence":"additional","affiliation":[{"name":"Centro Uruguayo de Imagenolog\u00eda Molecular (CUDIM)  Montevideo Uruguay"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana Laura","family":"Reyes","sequence":"additional","affiliation":[{"name":"Centro Uruguayo de Imagenolog\u00eda Molecular (CUDIM)  Montevideo Uruguay"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gianna","family":"Cecchetto","sequence":"additional","affiliation":[{"name":"\u00c1rea de Microbiolog\u00eda, Instituto de Qu\u00edmica Biol\u00f3gica, Facultad de Ciencias\u2010Departamento de Biociencias, Facultad de Qu\u00edmica Universidad de la Rep\u00fablica (Udelar)  Montevideo Uruguay"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,9,28]]},"reference":[{"key":"e_1_2_7_2_1","first-page":"20","article-title":"Technetium\u201099m\u2010human polyclonal IgG radiolabeled via the hydrazinonicotinamide derivative for imaging focal sites of infection in rats","volume":"31","author":"Abrams M. 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