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However, most of these pharmacological effects are only traceable to the crude form of the plant extract and not specific natural products. Phytochemical investigation of the methanol fraction of the methanol extract of the stem\u2010bark of <jats:italic>Alstonia boonei<\/jats:italic> led to the isolation and identification of 2\u2010methyl\u20103\u2010propylbutane\u20101,4\u2010diol. The structures were elucidated by the application of 1D\u2010, and 2D\u2010NMR spectroscopic analyses and by comparison with literature data. In this study, the membrane stabilizing activity, mitochondrial membrane permeability transition pore opening, cytochrome c release, mitochondrial ATPase activity, and prevention of mitochondrial lipid peroxidation activity of 2\u2010methyl\u20103\u2010propylbutane\u20101,4\u2010diol (MPBD) isolated from <jats:italic>A. boonei<\/jats:italic> were determined. The results showed that MPBD significantly (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05) prevented peroxidation of mitochondrial membrane lipids and hemolysis using both the heat\u2010induced and hypotonic solution\u2010induced membrane stabilization assays. On the contrary, the compound caused large amplitude swelling of rat liver mitochondria in the absence of calcium, significant (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05) cytochrome c release and enhancement of mitochondrial ATPase activity <jats:italic>in vitro<\/jats:italic>. Our findings suggest that MPBD showed characteristic biological properties useful in modulating cell death.<\/jats:p>","DOI":"10.1111\/cbdd.14168","type":"journal-article","created":{"date-parts":[[2022,11,3]],"date-time":"2022-11-03T03:54:34Z","timestamp":1667447674000},"page":"678-689","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["In vitro effects of 2\u2010methyl\u20103\u2010propylbutane\u20101,4\u2010diol purified from <i>Alstonia boonei<\/i> on erythrocyte membrane stabilization and mitochondrial membrane permeabilization"],"prefix":"10.1111","volume":"101","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8882-1601","authenticated-orcid":false,"given":"John Oludele","family":"Olanlokun","sequence":"first","affiliation":[{"name":"Laboratories for Biomembrane Research and Biotechnology, Department of Biochemistry College of Medicine, University of Ibadan  Ibadan 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