{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T23:09:48Z","timestamp":1792019388966,"version":"4.4.0"},"reference-count":41,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2023,2,21]],"date-time":"2023-02-21T00:00:00Z","timestamp":1676937600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chem Biol Drug Des"],"published-print":{"date-parts":[[2023,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Herein, two iridoid glucosides aucubin (1) and ajugol (2), and two phenyl ethanoids, verbascoside (3) and poliumoside (4) were isolated from the methanol extract of the aerial parts of\n                    <jats:italic>Verbascum speciosum<\/jats:italic>\n                    and used to study about their anticancer activity for the first time. The structures of all compounds were elucidated using spectroscopic data (IR, 1D and 2D NMR, LC\u2010TOF\/MS). Antiproliferative activities of Aucubun (\n                    <jats:bold>1<\/jats:bold>\n                    ) and Verbascoside (3) were tested against A\u2010549 (human colon cancer), MDA\u2010MD\u2010453 (human breast cancer) and 3T3\u2010L1 (mouse fibroblast)cell lines by XTT assay. In addition, the anticarcer mechanism of action of aucubin (1) was investigated on MDA\u2010MB\u2010453 cells for the first time. XTT result showed that both applied compounds exhibited antiproliferative effect at different dose ranges depending on the cancer type, as well as selectivity between cancer and healty cell lines. Flow cytometry analyzes revealed that aucubin (1) exerts its cytotoxic effect in MDA\u2010MB\u2010453 cells by directing cells to early apoptosis and inhibiting the P13K\/AKT signaling pathway.\n                  <\/jats:p>","DOI":"10.1111\/cbdd.14211","type":"journal-article","created":{"date-parts":[[2023,2,9]],"date-time":"2023-02-09T03:15:37Z","timestamp":1675912537000},"page":"1273-1282","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Isolation, characterization and anticancer activity of secondary metabolites from\n                    <i>Verbascum speciosum<\/i>"],"prefix":"10.1111","volume":"101","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6579-4273","authenticated-orcid":false,"given":"Serpil","family":"Demirci","sequence":"first","affiliation":[{"name":"Department of Medical Services and Techniques, Vocational High School of Health Services Giresun University  Giresun T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cemalettin","family":"Alp","sequence":"additional","affiliation":[{"name":"Department of Chemistry Faculty of Arts and Science, Erzincan Binali Y\u0131ld\u0131r\u0131m University  Erzincan T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H\u00fcseyin","family":"Ak\u015fit","sequence":"additional","affiliation":[{"name":"Department of Analytical Chemistry Faculty of Pharmacy, Erzincan Binali Y\u0131ld\u0131r\u0131m University  Erzincan T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yakup","family":"Uluta\u015f","sequence":"additional","affiliation":[{"name":"Department of Chemistry Faculty of Arts and Science, Erzincan Binali Y\u0131ld\u0131r\u0131m University  Erzincan T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmet","family":"Altay","sequence":"additional","affiliation":[{"name":"Department of Chemistry Faculty of Arts and Science, Erzincan Binali Y\u0131ld\u0131r\u0131m University  Erzincan T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Esma","family":"Yeni\u00e7eri","sequence":"additional","affiliation":[{"name":"Erzincan Binali Y\u0131ld\u0131r\u0131m University Institute of Science and Technology  Erzincan T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ekrem","family":"K\u00f6ksal","sequence":"additional","affiliation":[{"name":"Department of Chemistry Faculty of Arts and Science, Erzincan Binali Y\u0131ld\u0131r\u0131m University  Erzincan T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nurettin","family":"Yayl\u0131","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, School of Medicine Karadeniz Technical University  Trabzon T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,2,21]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2010.05.059"},{"issue":"2","key":"e_1_2_7_3_1","first-page":"227","article-title":"Iridoid and phenylethanoid glycosides from Verbascum lasianthum","volume":"28","author":"Akdemir Z. \u015e.","year":"2004","journal-title":"Turkish Journal of Chemistry"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.32571\/ijct.577183"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1080\/13813455.2019.1662454"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfbc.12908"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbdv.202200348"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sajb.2022.08.032"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0031\u20109422(00)82429\u20102"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mrgentox.2014.07.002"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010018\u201035083\u20102"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jphotochem.2021.113613"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12013\u2010021\u201000984\u2010z"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/963457"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.25135\/rnp.370.2210.2598"},{"key":"e_1_2_7_16_1","first-page":"193","volume-title":"Scrophulariaceae. 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