{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,27]],"date-time":"2026-04-27T21:25:16Z","timestamp":1777325116587,"version":"3.51.4"},"reference-count":24,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2023,3,17]],"date-time":"2023-03-17T00:00:00Z","timestamp":1679011200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chem Biol Drug Des"],"published-print":{"date-parts":[[2023,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Panax notoginseng<\/jats:italic> saponins (PNS), the active ingredients of the traditional Chinese medicine <jats:italic>Panax notoginseng,<\/jats:italic> have strong neuroprotective and anti\u2010platelet aggregation effects. To investigate whether PNS can promote hair follicle growth in C57BL\/6J mice, the optimal concentration of PNS was initially determined, followed by clarification of the mechanism underlying their effects. Twenty\u2010five male C57BL\/6J mice had the hair on a 2\u2009\u00d7\u20093\u00a0cm<jats:sup>2<\/jats:sup> area of the dorsal skin shaved and were equally divided into five groups: control group, 5% minoxidil (MXD) group, and three PNS treatment groups [2% (10\u00a0mg\/kg), 4% (20\u2009mg\/kg), and 8% (40\u2009mg\/kg) PNS]. They were then intragastrically administered the corresponding drugs for 28\u2009days. The effects of PNS on C57BL\/6J mice were analyzed by subjecting their dorsal depilated skin samples to different assessments, including hematoxylin and eosin staining, immunohistochemistry, immunofluorescence, quantitative real\u2010time polymerase chain reaction (qRT\u2010PCR), and Western blotting (WB). The group with 8% PNS exhibited the largest number of hair follicles from 14\u2009days onwards. Compared with the control group, the number of hair follicles increased significantly in the mice treated with 8% PNS and 5% MXD, which significantly increased in a PNS\u2010dose\u2010dependent manner. Immunohistochemistry and immunofluorescence results revealed that treatment with 8% PNS activated the metabolism of hair follicle cells, with them showing higher rates of proliferation and apoptosis than those in the normal group. In qRT\u2010PCR and WB analysis, the expression of \u03b2\u2010catenin, Wnt10b, and LEF1 was upregulated in the PNS and MDX groups compared with that in the control group. Examination of the WB bands revealed that the greatest inhibitory effect of Wnt5a occurred in mice in the 8% PNS group. PNS may promote the growth of hair follicles in mice, with 8% PNS demonstrating the strongest effect. The mechanism behind this may be related to the Wnt\/\u03b2\u2010catenin signaling pathway.<\/jats:p>","DOI":"10.1111\/cbdd.14224","type":"journal-article","created":{"date-parts":[[2023,3,3]],"date-time":"2023-03-03T13:20:05Z","timestamp":1677849605000},"page":"1416-1424","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["<i>Panax notoginseng<\/i> saponins promote hair follicle growth in mice via Wnt\/<scp>\u03b2\u2010Catenin<\/scp> signaling pathway"],"prefix":"10.1111","volume":"101","author":[{"given":"Dan","family":"Huang","sequence":"first","affiliation":[{"name":"Department of Plastic and Aesthetic Surgery The First Affiliated Hospital of Guangxi Medical University  Nanning China"},{"name":"Department of Plastic Surgery Zhejiang Putuo Hospital  Zhoushan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhen\u2010yu","family":"Gong","sequence":"additional","affiliation":[{"name":"Department of Burn, Plastic and Aesthetic Surgery Affiliated Hospital of Guilin Medical University  Guilin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shun\u2010cai","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Burn, Plastic and Aesthetic Surgery Affiliated Hospital of Guilin Medical University  Guilin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xian\u2010ping","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Plastic and Aesthetic Surgery The First Affiliated Hospital of Guangxi Medical University  Nanning China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kaung Myat","family":"Kyaw","sequence":"additional","affiliation":[{"name":"Department of Plastic and Aesthetic Surgery The First Affiliated Hospital of Guangxi Medical University  Nanning China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4242-5587","authenticated-orcid":false,"given":"Bo\u2010jie","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Plastic and Aesthetic Surgery The First Affiliated Hospital of Guangxi Medical University  Nanning China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,3,17]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2017.02.054"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/jdv.17079"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13555\u2010018\u20100278\u20106"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/lsm.22170"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2006.03.030"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms19092703"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21144915"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21082702"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00403\u2010017\u20101759\u20107"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1517\/14656566.2015.1084501"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.colsurfb.2018.11.067"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1080\/19768354.2022.2085790"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1155\/2021\/6667011"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1155\/2022\/2588608"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/jocd.12446"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/jocd.14537"},{"issue":"6","key":"e_1_2_10_18_1","first-page":"371","article-title":"Hair loss: Common causes and treatment","volume":"96","author":"Phillips T. 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