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Chuan Xiong is a traditional Chinese medicine and ligustrazine is a natural compound from it. Ligustrazine improves coronary blood flow and reduces cardiac ischemia in animals through Ca<jats:sup>2+<\/jats:sup> and ATP\u2010dependent vascular relaxation. It also decreases the platelets' bioactivity and reduces reactive oxygen species formation. We hypothesized that ligustrazine could protect liver by decreasing the inflammation response, protein production, and apoptosis in THS rats. Ligustrazine at doses of 100 and 1000\u2009\u03bcg\/mL was administrated in Kupffer cells isolated from THS rats. The protein expressions were detected via western blot. The THS showed increased inflammation response proteins, mitochondria\u2010dependent apoptosis proteins, and had a compensation effect on the Akt pathway. After ligustrazine treatment, the hemorrhagic shock Kupffer cells decreased inflammatory response and mitochondria\u2010dependent apoptosis and promoted a more compensative effect of the Akt pathway. It suggests ligustrazine reduces inflammation response and mitochondria\u2010dependent apoptosis induced by THS in liver Kupffer cells and promotes more survival effects by elevating the Akt pathway. These findings demonstrate the beneficial effects of ligustrazine against THS\u2010induced hepatic injury, and ligustrazine could be a potential medication to treat the liver injury caused by THS.<\/jats:p>","DOI":"10.1111\/cbdd.14327","type":"journal-article","created":{"date-parts":[[2023,8,24]],"date-time":"2023-08-24T01:15:20Z","timestamp":1692839720000},"page":"1399-1408","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Ligustrazine improves the compensative effect of Akt survival signaling to protect liver Kupffer cells in trauma\u2010hemorrhagic shock rats"],"prefix":"10.1111","volume":"102","author":[{"given":"Ray\u2010Jade","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Surgery, School of Medicine, College of Medicine Taipei Medical University  Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming\u2010Cheng","family":"Chen","sequence":"additional","affiliation":[{"name":"Division of Colorectal Surgery, Department of Surgery Taichung Veterans General Hospital  Taichung Taiwan"},{"name":"Faculty of Medicine National Yang Ming Chiao Tung University  Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruce Chi\u2010Kang","family":"Tsai","sequence":"additional","affiliation":[{"name":"Cardiovascular and Mitochondrial Related Disease Research Center Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation  Hualien Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rakesh","family":"Roy","sequence":"additional","affiliation":[{"name":"Cardiovascular and Mitochondrial Related Disease Research Center Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation  Hualien Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi\u2010Ru","family":"Chang","sequence":"additional","affiliation":[{"name":"Department of Surgery, School of Medicine, College of Medicine Taipei Medical University  Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tso\u2010Fu","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Hematology and Oncology Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation  Hualien Taiwan"},{"name":"School of Medicine Tzu Chi University, Buddhist Tzu Chi Medical Foundation  Hualien Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei\u2010Wen","family":"Kuo","sequence":"additional","affiliation":[{"name":"Department of Biological Science and Technology China Medical University  Taichung Taiwan"},{"name":"Ph.D. Program for Biotechnology Industry China Medical University  Taichung Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chia\u2010Hua","family":"Kuo","sequence":"additional","affiliation":[{"name":"Department of Sports Sciences University of Taipei  Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chun\u2010Hsu","family":"Yao","sequence":"additional","affiliation":[{"name":"Department of Biomedical Imaging and Radiological Science China Medical University  Taichung Taiwan"},{"name":"School of Chinese Medicine China Medical University  Taichung Taiwan"},{"name":"Biomaterials Translational Research Center China Medical University Hospital  Taichung Taiwan"},{"name":"Department of Bioinformatics and Medical Engineering Asia University  Taichung Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chi\u2010Cheng","family":"Li","sequence":"additional","affiliation":[{"name":"School of Medicine Tzu Chi University, Buddhist Tzu Chi Medical Foundation  Hualien Taiwan"},{"name":"Center of Stem Cell &amp; 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