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The polarization of microglia affects IS process. This research is powered to probe whether the molecular mechanism of JAS for IS treatment is coupled with microglia polarization. IS modeling in mice was accomplished by middle cerebral artery occlusion (MCAO) and model mice were injected with 25 and 50\u2009mg\/mL JAS, followed by determination of infarct volume, brain water content, and histological changes in mouse brains. The microglia modeling was performed by 1\u2010h oxygen\u2013glucose deprivation and 24\u2010h reoxygenation. Oxygen\u2013glucose deprivation\/reoxygenation (OGD\/R)\u2010induced microglia were treated with JAS and transfected with Per\u2010Arnt\u2010Sim kinase (PASK)\u2010overexpressing plasmid, subsequent to which cell viability and lactate dehydrogenase (LDH) level were determined. The mRNA or protein expressions of examined genes in microglia and brain tissues were detected by quantitative real\u2010time polymerase chain reaction or western blot. MCAO\u2010induced massive infarction, edema, and injury in mouse brain tissues, upregulated interleukin\u20101 beta (IL\u20101\u03b2), Fc\u03b3RIIB (CD32), tumor necrosis factor alpha (TNF\u2010\u03b1), PASK, p\u2010eukaryotic elongation factor 1A1 (EEF1A1), and p\u2010EEF1A1\/EEF1A1 levels, but downregulated mannose receptor 1 (CD206), arginase\u20101 (Arg\u20101) and interleukin\u201010 (IL\u201010), and EEF1A1 expressions, which was reversed by JAS. OGD\/R treatment decreased microglial viability as well as expressions of CD206, Arg\u20101, IL\u201010, and EEF1A1, yet increased cytotoxicity and levels of IL\u20101\u03b2, CD32, TNF\u2010\u03b1, PASK, p\u2010EEF1A1, and p\u2010EEF1A1\/EEF1A1, which was reversed by JAS. PASK overexpression reversed the effects of JAS on microglia. JAS reduces IS injury by regulating microglia polarization via PASK\u2010EEF1A1 axis.<\/jats:p>","DOI":"10.1111\/cbdd.14354","type":"journal-article","created":{"date-parts":[[2023,9,25]],"date-time":"2023-09-25T02:25:25Z","timestamp":1695608725000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Jasminoidin reduces ischemic stroke injury by regulating microglia polarization via <scp>PASK\u2010EEF1A1<\/scp> axis"],"prefix":"10.1111","volume":"103","author":[{"given":"Jinhan","family":"Wu","sequence":"first","affiliation":[{"name":"School of Medicine Hangzhou City University  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiqi","family":"Mao","sequence":"additional","affiliation":[{"name":"School of Medicine Hangzhou City University  Hangzhou 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