{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T14:35:33Z","timestamp":1789569333304,"version":"build-2803163510"},"reference-count":57,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,2,12]],"date-time":"2024-02-12T00:00:00Z","timestamp":1707696000000},"content-version":"vor","delay-in-days":11,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chem Biol Drug Des"],"published-print":{"date-parts":[[2024,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Bladder cancer (BLCA), a common primary malignancy, exhibits resistance to conventional chemotherapeutic agents. Sophoridine (SR) is a quinoline alkaloid derived from the traditional Chinese herb <jats:italic>Sophora alopecuroides<\/jats:italic> L., which belongs to the legume family Sophoraceae. SR is reported to exert growth\u2010inhibitory effects against several cancers. However, the mechanisms underlying the growth\u2010inhibitory effects of SR on BLCA have not been elucidated. This study performed molecular and cellular experiments to verify the growth\u2010inhibitory effects of SR on BLCA and the underlying mechanisms. SR inhibited cell proliferation and promoted apoptosis and G1\u2010phase arrest through the PI3K\/AKT\/FoxO3a signaling pathway. More interestingly, the effects of SR can be attributed to the accumulation of reactive oxygen species (ROS) in\u00a0vivo. ROS may be the upstream factor of this pathway. Additionally, SR inhibited the migration and invasion of BLCA cells in a concentration\u2010dependent or time\u2010dependent manner. This is the first study to demonstrate the ROS\u2010dependent PI3K\/AKT\/FoxO3a pathway\u2010mediated anticancer effect of SR and the anticancer mechanism of SR in BLCA. The correlation between SR\u2010induced ROS\u2010dependent cell proliferation inhibition, apoptosis, cell cycle arrest, and PI3K\/AKT\/FoxO3a suggests that SR is a promising novel therapeutic for BLCA.<\/jats:p>","DOI":"10.1111\/cbdd.14476","type":"journal-article","created":{"date-parts":[[2024,2,13]],"date-time":"2024-02-13T01:34:14Z","timestamp":1707788054000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Network pharmacology and in\u00a0vitro experiments reveal sophoridine\u2010induced apoptosis and <scp>G<sub>1<\/sub><\/scp> phase arrest via <scp>ROS<\/scp>\u2010dependent <scp>PI3K<\/scp>\/Akt\/<scp>FoxO3a<\/scp> pathway activation in human bladder cancer cells"],"prefix":"10.1111","volume":"103","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1389-7673","authenticated-orcid":false,"given":"Yao","family":"Su","sequence":"first","affiliation":[{"name":"College of Pharmacy Chengdu University  Chengdu 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