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This study investigated the role of sinigrin in oxidative stress using a CIR injury model. The effects of sinigrin were studied in middle cerebral artery occlusion (MCAO) rats and oxygen\u2013glucose deprivation\/reoxygenation (OGD\/R)\u2010injured SH\u2010SY5Y cells. Sinigrin treatment improved brain injury and neurological deficits induced by MCAO surgery in rats. Sinigrin inhibited apoptosis in brain tissues and SH\u2010SY5Y cells following OGD\/R induction. Additionally, sinigrin elevated the levels of superoxide dismutase (SOD), glutathione (GSH) and glutathione peroxidase (GSH\u2010Px) while reducing malondialdehyde (MDA) levels. Furthermore, sinigrin inhibited the toll\u2010like receptor 4 (TLR4)\/myeloid differentiation factor 88 (MyD88) signalling pathway. The anti\u2010apoptotic and antioxidant activities of sinigrin in OGD\/R\u2010injured SH\u2010SY5Y cells were reversed by TLR4 overexpression. In conclusion, sinigrin inhibits oxidative stress in CIR injury by suppressing the TLR4\/MyD88 signalling pathway.<\/jats:p>","DOI":"10.1111\/cbdd.14480","type":"journal-article","created":{"date-parts":[[2024,2,19]],"date-time":"2024-02-19T02:30:17Z","timestamp":1708309817000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Sinigrin improves cerebral ischaemia\u2010reperfusion injury by inhibiting the <scp>TLR4<\/scp> pathway\u2010mediated oxidative stress"],"prefix":"10.1111","volume":"103","author":[{"given":"Shenglong","family":"Guo","sequence":"first","affiliation":[{"name":"Department II of Neurology Shaanxi Provincial People's Hospital  Xi'an Shaanxi P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qi","family":"Lei","sequence":"additional","affiliation":[{"name":"Department II of Neurology Shaanxi Provincial People's Hospital  Xi'an Shaanxi P.R. 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