{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,22]],"date-time":"2026-02-22T09:32:20Z","timestamp":1771752740506,"version":"3.50.1"},"reference-count":40,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2024,5,29]],"date-time":"2024-05-29T00:00:00Z","timestamp":1716940800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chem Biol Drug Des"],"published-print":{"date-parts":[[2024,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Peimenine (PEI) is a steroid alkaloid substance isolated from <jats:italic>Fritillaria thunbergii<\/jats:italic> bulbs. It has various pharmacological activities, such as relief from coughs and asthma, expectorant properties, antibacterial effects, sedative qualities, and anti\u2010inflammatory properties. Notably, PEI can effectively inhibit the proliferation and tumor formation of liver cancer and osteosarcoma cells by inducing autophagic cell death. However, the precise effect and mechanisms of PEI on urothelial bladder cancer (UBC) cells remain uncertain. Thus, this study aims to investigate the impact of PEI on UBC cells both in\u00a0vivo and in\u00a0vitro. The IC<jats:sub>50<\/jats:sub> values of BIU\u201087 and EJ\u20101 cells after 48\u2009h were 710.3 and 651.1\u2009\u03bcg\/mL, respectively. Additionally, PEI blocked the cell cycle in BIU\u201087 and EJ\u20101 cells during the G1 phase. Furthermore, it hindered the migration of BIU\u201087 and EJ\u20101 cells substantially. PEI significantly inhibited the tumor development of EJ\u20101 cells within the xenograft tumor model in\u00a0vivo. Mechanically, PEI augmented the protein and mRNA expression of <jats:italic>BIM<\/jats:italic>, <jats:italic>BAK1<\/jats:italic>, and <jats:italic>Cytochrome C<\/jats:italic> (<jats:italic>CYCS)<\/jats:italic> in UBC cells. Taken together, PEI suppressed the proliferation of UBC cells both in\u00a0vitro and in\u00a0vivo by inducing cell death and cell cycle arrest, suggesting that PEI could be applied in the treatment of UBC.<\/jats:p>","DOI":"10.1111\/cbdd.14528","type":"journal-article","created":{"date-parts":[[2024,5,30]],"date-time":"2024-05-30T02:39:24Z","timestamp":1717036764000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Peimenine unleashes therapeutic promise in urothelial bladder cancer: inhibition of proliferation, induction of cell death and modulation of key pathways"],"prefix":"10.1111","volume":"103","author":[{"given":"Zhao","family":"Yang","sequence":"first","affiliation":[{"name":"College of Life Science and Technology, Innovation Center of Molecular Diagnostics Beijing University of Chemical Technology  Beijing China"},{"name":"College of Life Science and Technology, Key Laboratory of Protection and Utilization 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