{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T12:19:35Z","timestamp":1753877975875,"version":"3.41.2"},"reference-count":44,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,7,16]],"date-time":"2024-07-16T00:00:00Z","timestamp":1721088000000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chem Biol Drug Des"],"published-print":{"date-parts":[[2024,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Pterygium is a frequent eye surface condition that is characterized by a high rate of proliferation, fibrovascular development, cellular migration, corneal infiltration, and angiogenesis. We investigated that ex\u00a0vivo primary pterygium and conjunctival cell cultures were generated to analyze the effect of trehalose on cellular proliferation. After trehalose treatment, we performed microarray analysis to evaluate changes in the mRNA profile. We analyzed gene ontology (GO) and KEGG pathways to identify hub genes that changed expression levels after treatment and were associated with pterygium development. We selected three genes to verify their expression levels using qRT\u2013PCR. The study also evaluated the impact of trehalose treatment on cell migration through a wound\u2010healing assay. Our results suggested that pterygium cell proliferation was inhibited in a dose\u2010dependent manner by trehalose. 2354 DEG were identified in pterygium and conjunctiva cells treated with trehalose compared to untreated groups. Functional enrichment analysis showed that differentially expressed mRNAs are involved in proliferation, vasculature development, and cell migration. We identified ten hub genes including upregulated (<jats:italic>RANBP3L, SLC5A3, RERG, ANKRD1, DHCR7, RAB27B, GPRC5B, MSMO1, ASPN, DRAM1<\/jats:italic>) and downregulated (<jats:italic>TNC, PTGS2, GREM2, NPTX1, NR4A1, HMOX1, CXCL12, IL6, MYH2, TXNIP<\/jats:italic>). Microarray analysis and functional investigations suggest that trehalose affects the pathogenesis of pterygium by modifying the expression of genes involved in crucial pathways related to cell function.<\/jats:p>","DOI":"10.1111\/cbdd.14571","type":"journal-article","created":{"date-parts":[[2024,7,17]],"date-time":"2024-07-17T02:08:06Z","timestamp":1721182086000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Integrative analysis of ex\u00a0vivo studies and microarray reveals the novel inhibitor effects of trehalose on the pathogenesis of pterygium"],"prefix":"10.1111","volume":"104","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-4917-1205","authenticated-orcid":false,"given":"Yasin","family":"Durkal","sequence":"first","affiliation":[{"name":"Department of Ophthalmology, Faculty of Medicine Pamukkale University  Denizli Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9341-7945","authenticated-orcid":false,"given":"Kubilay","family":"\u0130nci","sequence":"additional","affiliation":[{"name":"Department of Cancer Molecular Biology Institution of Health Sciences, Pamukkale University  Denizli Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0537-9032","authenticated-orcid":false,"given":"Onur","family":"Tokgun","sequence":"additional","affiliation":[{"name":"Department of Medical Genetics, Faculty of Medicine Pamukkale University  Denizli Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0187-5381","authenticated-orcid":false,"given":"Ugur","family":"Yilmaz","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Faculty of Medicine Pamukkale University  Denizli Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Banu Candan","family":"Y\u0131lmaz","sequence":"additional","affiliation":[{"name":"Department of Cancer Molecular Biology Institution of Health Sciences, Pamukkale University  Denizli Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,7,16]]},"reference":[{"doi-asserted-by":"publisher","key":"e_1_2_8_2_1","DOI":"10.4103\/2230\u20108229.121980"},{"doi-asserted-by":"publisher","key":"e_1_2_8_3_1","DOI":"10.3980\/j.issn.2222\u20103959.2014.03.31"},{"doi-asserted-by":"publisher","key":"e_1_2_8_4_1","DOI":"10.1016\/j.drudis.2022.103416"},{"doi-asserted-by":"publisher","key":"e_1_2_8_5_1","DOI":"10.1007\/s10792\u2010021\u201001869\u2010z"},{"doi-asserted-by":"publisher","key":"e_1_2_8_6_1","DOI":"10.7150\/jca.73112"},{"doi-asserted-by":"publisher","key":"e_1_2_8_7_1","DOI":"10.1007\/s00417\u2010011\u20101676\u2010y"},{"doi-asserted-by":"publisher","key":"e_1_2_8_8_1","DOI":"10.1186\/s13046\u2010021\u201001982\u2010y"},{"doi-asserted-by":"publisher","key":"e_1_2_8_9_1","DOI":"10.1038\/s41433\u2010020\u20100786\u20103"},{"doi-asserted-by":"publisher","key":"e_1_2_8_10_1","DOI":"10.1136\/bjo.77.11.734"},{"doi-asserted-by":"publisher","key":"e_1_2_8_11_1","DOI":"10.1038\/s41598\u2010021\u201004248\u2010x"},{"doi-asserted-by":"publisher","key":"e_1_2_8_12_1","DOI":"10.1111\/jcmm.16024"},{"doi-asserted-by":"publisher","key":"e_1_2_8_13_1","DOI":"10.1038\/s41419\u2010020\u201002979\u20109"},{"doi-asserted-by":"publisher","key":"e_1_2_8_14_1","DOI":"10.1007\/s10792\u2010016\u20100358\u20105"},{"doi-asserted-by":"publisher","key":"e_1_2_8_15_1","DOI":"10.1111\/ceo.13351"},{"doi-asserted-by":"publisher","key":"e_1_2_8_16_1","DOI":"10.1038\/sj.eye.6700738"},{"doi-asserted-by":"publisher","key":"e_1_2_8_17_1","DOI":"10.18240\/ijo.2018.05.01"},{"doi-asserted-by":"publisher","key":"e_1_2_8_18_1","DOI":"10.1167\/iovs.13\u201012141"},{"doi-asserted-by":"publisher","key":"e_1_2_8_19_1","DOI":"10.1093\/nar\/gkw377"},{"doi-asserted-by":"publisher","key":"e_1_2_8_20_1","DOI":"10.3390\/biom10050809"},{"doi-asserted-by":"publisher","key":"e_1_2_8_21_1","DOI":"10.1016\/j.jtos.2022.08.002"},{"doi-asserted-by":"publisher","key":"e_1_2_8_22_1","DOI":"10.1038\/onc.2013.566"},{"doi-asserted-by":"publisher","key":"e_1_2_8_23_1","DOI":"10.1038\/onc.2013.566"},{"doi-asserted-by":"publisher","key":"e_1_2_8_24_1","DOI":"10.3892\/ijmm.2016.2641"},{"doi-asserted-by":"publisher","key":"e_1_2_8_25_1","DOI":"10.3109\/02713683.2013.823212"},{"doi-asserted-by":"publisher","key":"e_1_2_8_26_1","DOI":"10.3389\/fgene.2021.646550"},{"doi-asserted-by":"publisher","key":"e_1_2_8_27_1","DOI":"10.3390\/ijms24032047"},{"doi-asserted-by":"publisher","key":"e_1_2_8_28_1","DOI":"10.1038\/s41598\u2010017\u201007844\u2010y"},{"doi-asserted-by":"publisher","key":"e_1_2_8_29_1","DOI":"10.1111\/aos.13178"},{"doi-asserted-by":"publisher","key":"e_1_2_8_30_1","DOI":"10.18240\/ijo.2016.03.25"},{"doi-asserted-by":"crossref","unstructured":"Miyake T. 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