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In that sense, the labelling of antimicrobial peptides (AMPs) that are capable of binding specifically to the pathogenic microorganism which causes the infection, should provide a sufficiently specific agent, able to distinguish an infection from a sterile inflammation. Defensins are particularly interesting molecules with antimicrobial activity, the EcgDf1 defensin was identified from the genome of a Uruguayan native plant, <jats:italic>Erythrina crista<\/jats:italic>\u2010<jats:italic>galli<\/jats:italic>, the \u2018Ceibo\u2019 tree. Our group has previously reported a synthetic biologically active short analogue EcgDf21 (ERFTGGHCRGFRRRCFCTKHC) successfully labelled with <jats:sup>99m<\/jats:sup>Tc. Herein we present a shorter analogue which also preserves the \u03b3\u2010core domain, as a pharmacophore for a potential infection detection agent. This peptide was derivatized with the bifunctional chelating agent 1,4,7\u2010triazacyclononane\u20101,4,7\u2010triacetic acid (NOTA) through a lysine linker in the amino\u2010terminal group (NOTA\u2010KGHCRGFRRRC) and radiolabelled with <jats:sup>68<\/jats:sup>Ga ([<jats:sup>68<\/jats:sup>Ga]Ga\u2010NOTA\u2010K\u2010EcgDf1<jats:sub>(10)<\/jats:sub>). The [<jats:sup>68<\/jats:sup>Ga]Ga\u2010NOTA\u2010K\u2010EcgDf1<jats:sub>(10)<\/jats:sub> labelling procedure rendered a product with high radiochemical purity and stability in the labelling milieu. The Log P value indicated that the complex has a hydrophilic behaviour, confirmed by the biodistribution profile. The [<jats:sup>68<\/jats:sup>Ga]Ga\u2010NOTA\u2010K\u2010EcgDf1<jats:sub>(10)<\/jats:sub> complex demonstrated specific binding to cultures of <jats:italic>Candida albicans<\/jats:italic> and <jats:italic>Aspergillus niger<\/jats:italic>. Its biodistribution showed renal elimination and low accumulation in the rest of the body. It was possible to successfully differentiate sterile inflammation from infection by PET images in nude mice with a target\/non\u2010target ratio of 3.3 for <jats:italic>C<\/jats:italic>. <jats:italic>albicans<\/jats:italic> and 3.7 for <jats:italic>A. niger<\/jats:italic>, respectively.<\/jats:p>","DOI":"10.1111\/cbdd.14578","type":"journal-article","created":{"date-parts":[[2024,7,24]],"date-time":"2024-07-24T03:28:11Z","timestamp":1721791691000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Peptide derived from plant defensins: A promising <scp><sup>68<\/sup>Ga<\/scp> radiolabelled agent for diagnostic of infection foci in <scp>PET<\/scp>"],"prefix":"10.1111","volume":"104","author":[{"given":"Jessica","family":"Osorio","sequence":"first","affiliation":[{"name":"\u00c1rea de Radioqu\u00edmica, Departamento Estrella Campos, Facultad de Qu\u00edmica Universidad de la Rep\u00fablica (Udelar)  Montevideo Uruguay"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roberto Castro","family":"Rosas","sequence":"additional","affiliation":[{"name":"\u00c1rea de Microbiolog\u00eda, Instituto de Qu\u00edmica Biol\u00f3gica, Facultad de Ciencias\u2010Departamento de Biociencias, Facultad de Qu\u00edmica Universidad de la Rep\u00fablica (Udelar)  Montevideo Uruguay"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mariana Barraco","family":"Vega","sequence":"additional","affiliation":[{"name":"\u00c1rea de Microbiolog\u00eda, Instituto de Qu\u00edmica Biol\u00f3gica, Facultad de Ciencias\u2010Departamento de Biociencias, Facultad de Qu\u00edmica Universidad de la Rep\u00fablica (Udelar)  Montevideo Uruguay"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana Laura","family":"Reyes","sequence":"additional","affiliation":[{"name":"Departamento de Radiofarmacia Centro Uruguayo de Imagenolog\u00eda Molecular (CUDIM)  Montevideo Uruguay"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrea","family":"Paolino","sequence":"additional","affiliation":[{"name":"Departamento de Radiofarmacia Centro Uruguayo de Imagenolog\u00eda Molecular (CUDIM)  Montevideo Uruguay"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Florencia","family":"Men\u00e9ndez","sequence":"additional","affiliation":[{"name":"\u00c1rea de Radioqu\u00edmica, Departamento Estrella Campos, Facultad de Qu\u00edmica Universidad de la Rep\u00fablica (Udelar)  Montevideo Uruguay"},{"name":"\u00c1rea de Microbiolog\u00eda, Instituto de Qu\u00edmica Biol\u00f3gica, Facultad de Ciencias\u2010Departamento de Biociencias, Facultad de Qu\u00edmica Universidad de la Rep\u00fablica (Udelar)  Montevideo Uruguay"},{"name":"Departamento de Radiofarmacia Centro Uruguayo de Imagenolog\u00eda Molecular (CUDIM)  Montevideo Uruguay"},{"name":"Computacional Chemistry and Biological Group, DETEMA, Facultad de Qu\u00edmica Universidad de la Rep\u00fablica (Udelar)  Montevideo Uruguay"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mauricio","family":"Vega\u2010Teijido","sequence":"additional","affiliation":[{"name":"Computacional Chemistry and Biological Group, DETEMA, Facultad de Qu\u00edmica Universidad de la Rep\u00fablica (Udelar)  Montevideo Uruguay"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eduardo","family":"Savio","sequence":"additional","affiliation":[{"name":"Departamento de Radiofarmacia Centro Uruguayo de Imagenolog\u00eda Molecular (CUDIM)  Montevideo Uruguay"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Javier","family":"Giglio","sequence":"additional","affiliation":[{"name":"Departamento de Radiofarmacia Centro Uruguayo de Imagenolog\u00eda Molecular (CUDIM)  Montevideo Uruguay"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gianna","family":"Cecchetto","sequence":"additional","affiliation":[{"name":"\u00c1rea de Microbiolog\u00eda, Instituto de Qu\u00edmica Biol\u00f3gica, Facultad de Ciencias\u2010Departamento de Biociencias, Facultad de Qu\u00edmica Universidad de la Rep\u00fablica (Udelar)  Montevideo Uruguay"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4566-0814","authenticated-orcid":false,"given":"Mariella","family":"Ter\u00e1n","sequence":"additional","affiliation":[{"name":"\u00c1rea de Radioqu\u00edmica, Departamento Estrella Campos, Facultad de Qu\u00edmica Universidad de la Rep\u00fablica (Udelar)  Montevideo Uruguay"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,7,23]]},"reference":[{"issue":"3","key":"e_1_2_7_2_1","first-page":"245","article-title":"In vitro receptor binding assays: General methods and considerations","volume":"52","author":"Bigott\u2010Hennkens H. 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