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Herein, the effect of karanjin on sepsis\u2010stimulated ALI was investigated. We built a mouse model of lipopolysaccharide (LPS)\u2010stimulated ALI. The histopathological morphology of lung tissues was scrutinized by hematoxylin\u2013eosin (H&amp;E) staining. The lung injury score and lung wet\/dry weight ratio were detected. The myeloperoxidase (MPO) activity and malondialdehyde (MDA) content were scrutinized by commercial kits. Murine alveolar lung epithelial (MLE\u201012) cells were treated with LPS to mimic a cellular model of ALI. The cell viability was scrutinized by the CCK\u20108 assay. The contents of proinflammatory cytokines were scrutinized by qRT\u2010PCR and ELISA. The TLR4 and MyD88 contents were scrutinized by qRT\u2010PCR and western blotting. Results showed that karanjin alleviated LPS\u2010stimulated ALI in mice by inhibiting lung tissue lesions, edema, and oxidative stress. Moreover, karanjin inhibited LPS\u2010stimulated inflammation and TLR4 pathway activation in mice. However, treatment with GSK1795091, an agonist of TLR4, attenuated the effects of karanjin on LPS\u2010induced ALI. Furthermore, karanjin repressed LPS\u2010stimulated inflammatory response and TLR4 pathway activation in MLE\u201012 cells. Overexpression of TLR4 attenuated karanjin effects on LPS\u2010stimulated inflammatory responses in MLE\u201012 cells. In conclusion, karanjin repressed sepsis\u2010stimulated ALI in mice by suppressing the TLR4 pathway.<\/jats:p>","DOI":"10.1111\/cbdd.14579","type":"journal-article","created":{"date-parts":[[2024,7,17]],"date-time":"2024-07-17T02:08:08Z","timestamp":1721182088000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["The protective effect of karanjin against sepsis\u2010induced acute lung injury in mice is involved in the suppression of the <scp>TLR4<\/scp> pathway"],"prefix":"10.1111","volume":"104","author":[{"given":"Chujie","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Emergency Huai'an Second People's Hospital, The Affliated Huai'an Hospital of Xuzhou Medical University  Huai'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juncong","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Emergency Lianshui County People's Hospital  Huai'an 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