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Nanoemulsion based lipid nanoparticles containing demethylcantharidin (DNLNs) were prepared by loading nanoemulsions into lipid nanoparticles. The cells proliferation, apoptosis, cycle, and uptake were investigated by Cell counting kit\u20108 (CCK\u20108), flow cytometry, and in\u00a0situ fluorescence assays, respectively. Then, we established the H22 tumor\u2010bearing mouse model to evaluate the antitumor efficacy of DNLNs and further studied its organ toxicity and distribution. DNLNs significantly inhibited the proliferation and promoted apoptosis of H22 cells, and H22 cells could take up more DNLNs. Compared with DEM, DNLNs had certain tumor\u2010targeting properties, and the tumor inhibition rate increased by 23.24%. Moreover, DNLNs can increase white blood cell count and reduce organ toxicity. This study paves the way for nanoemulsion\u2010based lipid nanoparticle (NLNs)\u2010efficient DEM delivery to treat liver cancer.<\/jats:p>","DOI":"10.1111\/cbdd.14580","type":"journal-article","created":{"date-parts":[[2024,7,20]],"date-time":"2024-07-20T02:39:10Z","timestamp":1721443150000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Nanoemulsion based lipid nanoparticles for effective demethylcantharidin delivery to cure liver cancer"],"prefix":"10.1111","volume":"104","author":[{"given":"Zijun","family":"Yan","sequence":"first","affiliation":[{"name":"School of Pharmaceutical Sciences and Yunnan Key Laboratory of Pharmacology for Natural Products Kunming Medical University  Yunnan Kunming China"},{"name":"Department of Pharmacy Panzhihua Central Hospital  Sichuan Panzhihua 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