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However, current therapies are not effective in alleviating the progression of OA. Isoquercetin is a natural flavonoid glycoside compound that has various pharmacological effects including anticancer, anti\u2010diabetes and blood lipid regulation. Previous evidence suggests that isoquercetin has anti\u2010inflammatory properties in various diseases, but its effect on OA has not been investigated yet. In this study, through western bolt, qRT\u2010PCR and ELISA, it was found that isoquercetin could reduce the increase of ADAMTS5, MMP13, COX\u20102, iNOS and IL\u20106 induced by IL\u20101\u03b2, suggesting that isoquercetin could inhibit the inflammation and ECM degradation of chondrocytes. Through nuclear\u2010plasma separation technique, western blot and immunocytochemistry, it can be found that Nrf2 and NF\u2010\u03baB pathways are activated in this process, and isoquercetin may rely on this process to play its protective role. In\u00a0vivo, the results of X\u2010ray and SO staining show that intra\u2010articular injection of isoquercetin reduces the degradation of cartilage in the mouse OA model. In conclusion, the present work suggests that isoquercetin may benefit chondrocytes by regulating the Nrf2\/NF\u2010\u03baB signaling axis, which supports isoquercetin as a potential drug for the treatment of OA.<\/jats:p>","DOI":"10.1111\/cbdd.14620","type":"journal-article","created":{"date-parts":[[2024,9,10]],"date-time":"2024-09-10T02:05:06Z","timestamp":1725933906000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Isoquercetin Ameliorates Osteoarthritis via Nrf2\/<scp>NF<\/scp>\u2010<scp>\u03baB<\/scp> Axis: An In\u00a0Vitro and In\u00a0Vivo Study"],"prefix":"10.1111","volume":"104","author":[{"given":"He","family":"Yu","sequence":"first","affiliation":[{"name":"Department of Orthopaedics Zhejiang Hospital  Zhejiang Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junsheng","family":"Lou","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery, The 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