{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T03:16:37Z","timestamp":1783653397413,"version":"3.55.0"},"reference-count":31,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2024,12,13]],"date-time":"2024-12-13T00:00:00Z","timestamp":1734048000000},"content-version":"vor","delay-in-days":12,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2024,12,1]],"date-time":"2024-12-01T00:00:00Z","timestamp":1733011200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100015801","name":"Anhui Provincial Quality Engineering Project","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100015801","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003995","name":"Natural Science Foundation of Anhui Province","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003995","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chem Biol Drug Des"],"published-print":{"date-parts":[[2024,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Overexpression of c\u2010Myc is a key factor in the development of leukemia and other malignancies, highlighting the urgent need for novel drugs to inhibit c\u2010Myc protein levels. DNA G\u2010quadruplexes (G4) have emerged as potential regulatory targets for c\u2010Myc expression. Previous studies identified trovafloxacin, a topoisomerase II inhibitor, as a novel c\u2010Myc G4 stabilizer. In this study, virtual screening based on structural similarity led to the identification of nine derivatives of trovafloxacin, among which voreloxin exhibited potent cytotoxicity in multiple myeloma cells and showed promising therapeutic efficacy in leukemia cells. FRET assays demonstrated that voreloxin specifically stabilized the G4 structures of c\u2010Myc and Bcl\u20102, with minimal effects on the G4 structures of other oncogenes. Moreover, voreloxin significantly reduced the expression levels of c\u2010Myc and Bcl\u20102 in THP\u20101 and MOLM\u201013 cells. Molecular docking, molecular dynamics (MD) simulations, and MM\/GBSA calculations further confirmed the stable binding of voreloxin to both c\u2010Myc and Bcl\u20102 G4s, primarily driven by \u03c0\u2010\u03c0 stacking and hydrogen bonding interactions. These findings provide valuable insights for the development of G4\u2010targeting drugs for cancer therapy.<\/jats:p>","DOI":"10.1111\/cbdd.70034","type":"journal-article","created":{"date-parts":[[2024,12,14]],"date-time":"2024-12-14T02:15:12Z","timestamp":1734142512000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Discovery of Voreloxin as a Dual\u2010Selective Stabilizer for c\u2010Myc\/Bcl\u20102\u2009G\u2010Quadruplexes in Leukemia"],"prefix":"10.1111","volume":"104","author":[{"given":"Jiacheng","family":"Yin","sequence":"first","affiliation":[{"name":"Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy Xuzhou Medical University  Xuzhou Jiangsu P. R. 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