{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T13:33:16Z","timestamp":1783517596797,"version":"3.55.0"},"reference-count":54,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,1,16]],"date-time":"2025-01-16T00:00:00Z","timestamp":1736985600000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chem Biol Drug Des"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Ischemic stroke (IS) often causes fearful sequela, even death. Curcumin was beneficial to IS, but its underlying molecular mechanism is unclear. Mice were subjected to middle cerebral artery occlusion (MCAO) surgery, and BV\u20102 cells were treated with oxygen\u2013glucose deprivation\/reoxygenation (OGD\/R) induction to establish IS models in\u00a0vivo and in\u00a0vitro. Abundance of genes and proteins was determined using quantitative real\u2010time polymerase chain reaction (RT\u2010qPCR), immunofluorescence (IF), and western blot. Interleukin\u20101\u03b2 (IL\u20101\u03b2), interleukin\u20106 (IL\u20106), and interleukin\u201010 (IL\u201010) levels were analyzed using enzyme\u2010linked immunosorbent assay (ELISA). Modified neurological severity score (mNSS), corner test, foot fault test, adhesive removal test, and 2,3,5\u2010triphenyltetrazolium chloride (TTC) staining were applied to evaluate the brain injury of mice. The correlation between miR\u2010205\u20105p and Kruppel\u2010like factor 2 (KLF2) was affirmed using dual luciferase reporter assay. Our results revealed that curcumin alleviated brain damage in MCAO mice through driving microglia M2 polarization. Of note, curcumin resulted in decreased miR\u2010205\u20105p expression in MCAO mice. miR\u2010205\u20105p knockdown resulted in promoted microglia M2 polarization in OGD\/R conditions and achieved similar results to curcumin treatment in MCAO mice. Moreover, curcumin played a promoting role in microglia M2 polarization under OGD\/R conditions, while miR\u2010205\u20105p overexpression or KLF2 knockdown abolished these effects. On the mechanism, miR\u2010205\u20105p was a target of curcumin, and miR\u2010205\u20105p further interacted with KLF2 to inhibit activating transcription factor 2 (ATF2) expression. miR\u2010205\u20105p, decreased by curcumin, suppressed microglia M2 polarization to worsen IS injury through the mediating KLF2\/ATF2 axis.<\/jats:p>","DOI":"10.1111\/cbdd.70050","type":"journal-article","created":{"date-parts":[[2025,1,17]],"date-time":"2025-01-17T06:06:49Z","timestamp":1737094009000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Curcumin Regulates Microglia Polarization to Alleviate Ischemic Stroke by Targeting microRNA\u2010205\u20105p\/Kruppel\u2010Like Factor 2 (KLF2)\/Activating Transcription Factor 2 (ATF2) Axis"],"prefix":"10.1111","volume":"105","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-8327-5283","authenticated-orcid":false,"given":"Xiangyu","family":"Cao","sequence":"first","affiliation":[{"name":"Hunan University of Medicine  Huaihua China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingzi","family":"Pu","sequence":"additional","affiliation":[{"name":"Huaihua Hospital of Traditional Chinese Medicine  Huaihua China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,1,16]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.2174\/1871527313666141023094720"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.5853\/jos.2022.02327"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2020.00612"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2024.141193"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.STR.0000200561.69611.f8"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm8010122"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ebiom.2021.103444"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood\u20102006\u201007\u2010036020"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.12659\/msm.918237"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2020.576650"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12975\u2010018\u20100615\u20101"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/s1474\u20104422(10)70164\u20102"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2022.109611"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.202303930"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2020.11003"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/s1474\u20104422(19)30034\u20101"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2021.01.018"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11064\u2010010\u20100309\u2010x"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.2147\/ijn.S300379"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2023.115653"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41418\u2010023\u201001178\u20101"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnagi.2017.00233"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.00072"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2018.01.089"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.2174\/0929867328666210628141550"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1080\/15384101.2021.1889188"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dnarep.2021.103157"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jmedchem.6b00975"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pneurobio.2007.07.008"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2021.07.067"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.7224"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/cbdd.14439"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/biof.1066"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00712.2012"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12974\u2010016\u20100774\u20105"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1021\/acschemneuro.1c00153"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10741\u2010020\u201009928\u2010w"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1161\/cir.0000000000000950"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.24976\/Discov.Med.202335176.42"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2018.05.143"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1111\/imcb.12455"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tig.2016.02.001"},{"issue":"11","key":"e_1_2_9_44_1","first-page":"21071","article-title":"Elevated Expressions of Serum miR\u201015a, miR\u201016, and miR\u201017\u20105p Are Associated With Acute Ischemic Stroke","volume":"8","author":"Wu J.","year":"2015","journal-title":"International Journal of Clinical and Experimental Medicine"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1161\/jaha.119.012885"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1002\/iub.2357"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.imlet.2014.03.005"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1155\/2022\/1296816"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1002\/wrna.1686"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2019.09.020"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1155\/2021\/5548706"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2024.109156"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.7868"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0140\u20106736(19)30427\u20101"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.18632\/aging.202721"}],"container-title":["Chemical Biology &amp; Drug Design"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cbdd.70050","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,13]],"date-time":"2025-04-13T11:13:23Z","timestamp":1744542803000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/cbdd.70050"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1]]},"references-count":54,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2025,1]]}},"alternative-id":["10.1111\/cbdd.70050"],"URL":"https:\/\/doi.org\/10.1111\/cbdd.70050","archive":["Portico"],"relation":{},"ISSN":["1747-0277","1747-0285"],"issn-type":[{"value":"1747-0277","type":"print"},{"value":"1747-0285","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,1]]},"assertion":[{"value":"2024-09-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-01-04","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-01-16","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70050"}}