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The development of new drugs holds promise for improving the prognosis of this lethal disease. The possible anti\u2010neoplastic role of morusin was investigated both in\u00a0vitro and in\u00a0vivo. Through cell viability and colony formation assays, we observed that morusin inhibited the proliferation of gallbladder cancer cells in\u00a0vitro. Wound healing and transwell assays revealed that morusin impeded the migration and invasion of gallbladder cancer cells. Given the observed morphological changes, we examined epithelial\u2010mesenchymal transition (EMT) markers. Subsequent investigations demonstrated that morusin treatment, both in\u00a0vitro and in\u00a0vivo, downregulated the expression of phospho\u2010STAT3 (Signal transducer and activator of transcription 3) and HIF\u20101\u03b1 (Hypoxia\u2010inducible factor 1\u03b1) in gallbladder cancer cells. Furthermore, morusin effectively reversed EMT induced by phospho\u2010STAT3 or HIF\u20101\u03b1. Morusin has a reversing effect on the EMT of gallbladder cancer cells by modulating STAT3\/HIF\u20101\u03b1 signaling.<\/jats:p>","DOI":"10.1111\/cbdd.70054","type":"journal-article","created":{"date-parts":[[2025,1,18]],"date-time":"2025-01-18T08:10:50Z","timestamp":1737187850000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Morusin Reverses Epithelial\u2010Mesenchymal Transition in Gallbladder Cancer Cells by Regulating <scp>STAT3<\/scp>\/<scp>HIF<\/scp>\u20101\u03b1 Signaling"],"prefix":"10.1111","volume":"105","author":[{"given":"Lichao","family":"Ji","sequence":"first","affiliation":[{"name":"Department of Hepato\u2010Biliary\u2010Pancreatic Surgery Shaoxing People's Hospital  Shaoxing People's Republic of China"},{"name":"School of Medicine Shaoxing University  Shaoxing People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yingjie","family":"Chai","sequence":"additional","affiliation":[{"name":"Department of Hepato\u2010Biliary\u2010Pancreatic Surgery Shaoxing People's Hospital  Shaoxing People's Republic of China"},{"name":"Department of General Surgery Haining People's Hospital  Haining People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chenhao","family":"Tong","sequence":"additional","affiliation":[{"name":"Department of Hepato\u2010Biliary\u2010Pancreatic Surgery Shaoxing People's Hospital  Shaoxing People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanxin","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Hepato\u2010Biliary\u2010Pancreatic Surgery Shaoxing People's Hospital  Shaoxing People's Republic of China"},{"name":"School of Medicine Shaoxing University  Shaoxing People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiandong","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Hepato\u2010Biliary\u2010Pancreatic Surgery Shaoxing People's Hospital  Shaoxing People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Baochun","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Hepato\u2010Biliary\u2010Pancreatic Surgery Shaoxing People's Hospital  Shaoxing People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianhua","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Hepato\u2010Biliary\u2010Pancreatic Surgery Shaoxing People's Hospital  Shaoxing People's Republic of China"},{"name":"School of Medicine Shaoxing University  Shaoxing People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,1,18]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2023.115533"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2024.107675"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41389\u2010022\u201000423\u20105"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.7150\/jca.35631"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms160921643"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.2147\/DDDT.S138320"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v21.i43.12211"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13046\u2010024\u201002949\u20105"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.3892\/ol.2017.6006"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008\u20105472.CAN\u201022\u20101559"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2018.11.006"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2020.183503"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcancer.2021.05.020"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2008.05.023"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2018.07.009"},{"issue":"1","key":"e_1_2_8_17_1","first-page":"289","article-title":"Morusin Induces Cell Death Through Inactivating STAT3 Signaling in Prostate Cancer Cells","volume":"5","author":"Lim S. 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