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The two rL\u2010A9 peptide variants, HYNIC\u2010rL\u2010A9 and HYNIC\u2010PEG<jats:sub>12<\/jats:sub>\u2010rL\u2010A9 were manually synthesized by solid phase methodology. <jats:sup>99m<\/jats:sup>Tc\u2010labelling of peptides was accomplished using EDDA and tricine as co\u2010ligands. [<jats:sup>99m<\/jats:sup>Tc]Tc\u2010HYNIC\u2010rL\u2010A9 showed poor uptake in HER2\u2010expressing human breast carcinoma SKBR3 cells whereas the PEGylated counterpart [<jats:sup>99m<\/jats:sup>Tc]Tc\u2010HYNIC\u2010PEG<jats:sub>12<\/jats:sub>\u2010rL\u2010A9 demonstrated high specific cellular uptake (3.01%\u2009\u00b1\u20090.14%) and low nanomolar binding affinity (<jats:italic>K<\/jats:italic><jats:sub><jats:italic>d<\/jats:italic><\/jats:sub>\u2009=\u200917.11\u2009\u00b1\u20097.63\u2009nM). Tumour uptake (SKBR3) of [<jats:sup>99m<\/jats:sup>Tc]Tc\u2010HYNIC\u2010PEG<jats:sub>12<\/jats:sub>\u2010rL\u2010A9 was higher at 1 and 3\u2009h in comparison to the non\u2010PEGylated radiopeptide. Blocking studies led to 70% reduction in accumulation of radioactivity in the tumour indicating specificity of the radiopeptide. Introduction of polyethylene glycol (PEG<jats:sub>12<\/jats:sub>) as pharmacokinetic modifier led to significantly improved biological profile of the [<jats:sup>99m<\/jats:sup>Tc]Tc\u2010HYNIC\u2010labelled rL\u2010A9 peptide conjugate.<\/jats:p>","DOI":"10.1111\/cbdd.70064","type":"journal-article","created":{"date-parts":[[2025,2,19]],"date-time":"2025-02-19T09:44:28Z","timestamp":1739958268000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["<scp><sup>99m<\/sup>Tc<\/scp>\u2010<scp>HYNIC PEGylated<\/scp> Peptide Probe Targeting <scp>HER2<\/scp>\u2010Expression in Breast Cancer"],"prefix":"10.1111","volume":"105","author":[{"given":"Sushree Arpitabala","family":"Yadav","sequence":"first","affiliation":[{"name":"Radiopharmaceuticals Division Bhabha Atomic Research Centre  Mumbai India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V. Kusum","family":"Vats","sequence":"additional","affiliation":[{"name":"Radiopharmaceuticals Division Bhabha Atomic Research Centre  Mumbai India"},{"name":"Homi Bhabha National Institute  Mumbai India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sonal","family":"Gupta","sequence":"additional","affiliation":[{"name":"Radiopharmaceuticals Division Bhabha Atomic Research Centre  Mumbai India"},{"name":"KET's V. G. Vaze College Mumbai University  Mumbai India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Krishna Mohan","family":"Repaka","sequence":"additional","affiliation":[{"name":"Board of Radiation and Isotope Technology  Mumbai India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0676-6157","authenticated-orcid":false,"given":"Drishty","family":"Satpati","sequence":"additional","affiliation":[{"name":"Radiopharmaceuticals Division Bhabha Atomic Research Centre  Mumbai India"},{"name":"Homi Bhabha National Institute  Mumbai India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,2,19]]},"reference":[{"key":"e_1_2_12_2_1","first-page":"2022","article-title":"Technetium\u201099m\u2010Human Polyclonal IgG Radiolabeled via the Hydrazino Nicotinamide Derivative for Imaging Focal Sites of Infection in Rats","volume":"31","author":"Abrams M. 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