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Rutecarpine (RUT), a quinazolinocarboline alkaloid that is naturally occurring and present in Chinese medicinal herbs, has been shown to have anticancer properties in several cancer cell lines. However, the specific antitumor mechanisms of RUT in NSCLC remain unclear. This study demonstrates that RUT induces apoptosis and significantly reduces the viability of NSCLC cell lines. This effect is achieved by stimulating intracellular ROS production, leading to mitochondrial dysfunction. The decreased cell viability observed with RUT treatment is attributed to the elimination of ROS and apoptosis through the suppression of ROS by N\u2010acetylcysteine (NAC). Furthermore, RUT therapy elevated the production of CXCL10 and CCL5 in NSCLC cell lines and markedly activated the STING pathway in NSCLC cells. Mechanistically, RUT substantially decreased the levels of PD\u2010L1 protein in NSCLC cells. Notably, in\u00a0vivo experiments demonstrated that RUT significantly inhibits mouse NSCLC tumor growth in mice, exhibiting anti\u2010tumor activity by elevating CD8<jats:sup>+<\/jats:sup> T cells. These findings strongly support RUT as a promising anti\u2010cancer drug for NSCLC.<\/jats:p>","DOI":"10.1111\/cbdd.70070","type":"journal-article","created":{"date-parts":[[2025,3,12]],"date-time":"2025-03-12T01:14:00Z","timestamp":1741742040000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Rutecarpine Suppresses Non\u2010Small Cell Lung Cancer Progression Through Activating the <scp>STING<\/scp> Pathway and\u00a0Elevating <scp>CD8<\/scp>+ T Cells"],"prefix":"10.1111","volume":"105","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-9212-0124","authenticated-orcid":false,"given":"Ze\u2010Bo","family":"Jiang","sequence":"first","affiliation":[{"name":"Zhuhai Hospital of Integrated Traditional Chinese and Western Medicine  Zhuhai Guangdong China"},{"name":"Zhuhai Hospital Affiliated to Faculty of Chinese Medicine, Macau University of Science and Technology  Taipa Macau 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