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In this article, we present the synthesis of novel 3\u2032\u2010phenyl\u20101\u2010((1\u2010aryl\u20101<jats:italic>H<\/jats:italic>\u20101,2,3\u2010triazol\u20105\u2010yl)methyl)spiro[indoline\u20103,2\u2032\u2010oxiran]\u20102\u2010one derivatives by the regioselective reaction of sulfur ylides with 1,2,3\u2010triazole\u2010tethered isatins and their anticancer effects on hepatocellular carcinoma (HCC) cells HepG2 and HCCLM3. The cell viability assays indicated that <jats:bold>RR\u201001<\/jats:bold> and <jats:bold>RR\u201007<\/jats:bold> had emerged as the most potent cytotoxic agents on the tested cell lines. Colony formation and migration assay results confirmed the anticancer effects of these compounds by inhibiting the formation of colonies and migration. Further, nuclear fragmentation staining assay showed that the compounds induce apoptosis. Acridine orange staining assays showed that our lead candidates (<jats:bold>RR\u201001<\/jats:bold> and <jats:bold>RR\u201007<\/jats:bold>) induced autophagy in liver cancer cells.<\/jats:p>","DOI":"10.1111\/cbdd.70091","type":"journal-article","created":{"date-parts":[[2025,3,29]],"date-time":"2025-03-29T05:43:32Z","timestamp":1743227012000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["1,2,3\u2010Triazole Tethered Spiro[Indoline\u2010Oxirane] Derivatives Induce Anticancer Effects in Human Hepatoma Cells"],"prefix":"10.1111","volume":"105","author":[{"given":"Shashikala Mariswamy","family":"Rajesh","sequence":"first","affiliation":[{"name":"Department of Studies in Chemistry University of Mysore, Manasagangotri  Mysuru India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7071-6619","authenticated-orcid":false,"given":"Prasanna","family":"Doddakunche Shivaramu","sequence":"additional","affiliation":[{"name":"Department of Applied Sciences Visvesvaraya Technological University, Muddenahalli Campus  Chikkaballapur India"},{"name":"Department of Pharmacology Yong Loo Lin School of Medicine, National University of Singapore  Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chandra Sekhar","family":"Bhol","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Yong Loo Lin School of Medicine, National University of Singapore  Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toreshettahally R.","family":"Swaroop","sequence":"additional","affiliation":[{"name":"Department of Studies in Organic Chemistry University of Mysore, Manasagangotri  Mysuru India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Habbanakuppe D.","family":"Preetham","sequence":"additional","affiliation":[{"name":"Department of Studies in Organic Chemistry University of Mysore, Manasagangotri  Mysuru India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rajaghatta N.","family":"Suresh","sequence":"additional","affiliation":[{"name":"Department of Studies in Chemistry University of Mysore, Manasagangotri  Mysuru India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arunachalam","family":"Chinnathambi","sequence":"additional","affiliation":[{"name":"Department of Botany and Microbiology College of Science, King Saud University  Riyadh Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chandramohan","family":"Govindasamy","sequence":"additional","affiliation":[{"name":"Department of Community Health Sciences College of Applied Medical Sciences, King Saud University  Riyadh Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sulaiman Ali","family":"Alharbi","sequence":"additional","affiliation":[{"name":"Department of Botany and Microbiology College of Science, King Saud University  Riyadh Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Veeresha Gowda","family":"Shalini","sequence":"additional","affiliation":[{"name":"Department of Studies in Chemistry University of Mysore, Manasagangotri  Mysuru India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kwang Seok","family":"Ahn","sequence":"additional","affiliation":[{"name":"Department of Science in Korean Medicine Kyung Hee University  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shobith","family":"Rangappa","sequence":"additional","affiliation":[{"name":"Adichunchanagiri Institute for Molecular Medicine, Adichunchanagiri University  Nagamangala India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0572-6305","authenticated-orcid":false,"given":"Kanchugarakoppal S.","family":"Rangappa","sequence":"additional","affiliation":[{"name":"Institution of Excellence, University of Mysore, Manasagangotri  Mysuru India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,3,26]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1039\/b304625g"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.02607"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmc.2015.07.069"},{"key":"e_1_2_8_5_1","first-page":"741","article-title":"The Incidence of Multiple Primary Cancers. 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