{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T00:51:13Z","timestamp":1767919873213,"version":"3.49.0"},"reference-count":44,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,4,19]],"date-time":"2025-04-19T00:00:00Z","timestamp":1745020800000},"content-version":"vor","delay-in-days":18,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chem Biol Drug Des"],"published-print":{"date-parts":[[2025,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>This study aimed to investigate the protective mechanisms of dexmedetomidine (Dex) and thrombopoietin (TPO) against hypoxia\/reoxygenation (H\/R)\u2010induced myocardial injury. Human cardiomyocyte AC16 cells were subjected to hypoxic conditions and treated with Dex and TPO. Cellular responses, including proliferation, apoptosis, and autophagy, were assessed. RNA sequencing and bioinformatic analyses were conducted to identify differentially expressed genes, followed by functional pathway enrichment analysis. The results demonstrated that Dex and TPO significantly promoted cell proliferation, reduced apoptosis and autophagy, and inhibited caspase\u20103 activity and light chain 3B (LC3B) expression. Pathway enrichment analysis revealed the involvement of mitogen\u2010activated protein kinase (MAPK), transforming growth factor beta (TGF\u2010\u03b2), and tumor necrosis factor (TNF) signaling pathways. Although both treatments demonstrated overlapping effects, they also exhibited distinct gene regulation mechanisms. These findings suggested that Dex and TPO could mitigate H\/R\u2010induced myocardial injury through complex gene regulatory mechanisms, highlighting their potential as therapeutic strategies for myocardial ischemia\u2013reperfusion injury (MIRI).<\/jats:p>","DOI":"10.1111\/cbdd.70105","type":"journal-article","created":{"date-parts":[[2025,4,19]],"date-time":"2025-04-19T07:35:36Z","timestamp":1745048136000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Bioinformatic Analysis of the Protective Effects of Dexmedetomidine and Thrombopoietin Against Hypoxia\/Reoxygenation\u2010Induced Injury in <scp>AC16<\/scp> Cells"],"prefix":"10.1111","volume":"105","author":[{"given":"Cuiyan","family":"Xing","sequence":"first","affiliation":[{"name":"Department of Anesthesiology Shandong Provincial Hospital Affiliated to Shandong First Medical University  Jinan Shandong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mingyi","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology, School and Hospital of Stomatology Cheeloo College of Medicine, Shandong University  Jinan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoyang","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology Shandong Provincial Hospital Affiliated to Shandong First Medical University  Jinan Shandong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Benhang","family":"Gong","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology Shandong Provincial Hospital Affiliated to Shandong First Medical University  Jinan Shandong China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,4,19]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10495\u2010015\u20101203\u20104"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.4103\/1658\u2010354X.177340"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvm026"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.114.221747"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.24943"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12079\u2010018\u20100480\u20104"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00439\u2010013\u20101321\u20104"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1071924"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.12688\/f1000research.2\u2010188.v2"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1124\/pr.117.013896"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10557\u2010024\u201007664\u2010z"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1155\/2017\/1674613"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.2014.54"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12192\u2010023\u201001328\u2010x"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.26320"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2011.12.013"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2009.08.020"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.112.000936"},{"issue":"5","key":"e_1_2_10_20_1","first-page":"1106","article-title":"Influence of Dexmedetomidine to Cognitive Function During Recovery Period for Children With General Anesthesia","volume":"21","author":"Jia Z. M.","year":"2017","journal-title":"European Review for Medical and Pharmacological Sciences"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10557\u2010024\u201007579\u20109"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood\u20102004\u201008\u20103109"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2015.11.006"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12185\u2010013\u20101382\u20100"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.4097\/kja.19259"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp352"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1155\/2019\/7869318"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep42938"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1080\/10717544.2023.2298514"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1093\/abbs\/gmx120"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/390892"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp656"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.3390\/biomedicines12040802"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.25785"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.2023023559"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M007958200"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2017.00059"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11010\u2010024\u201005204\u20109"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkt216"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1111\/1440\u20101681.12711"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra071667"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.26355\/eurrev_201806_15183"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2014.05.005"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1038\/jcbfm.2010.171"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.25934"}],"container-title":["Chemical Biology &amp; Drug Design"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cbdd.70105","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,28]],"date-time":"2025-04-28T07:23:01Z","timestamp":1745824981000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/cbdd.70105"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,4]]},"references-count":44,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2025,4]]}},"alternative-id":["10.1111\/cbdd.70105"],"URL":"https:\/\/doi.org\/10.1111\/cbdd.70105","archive":["Portico"],"relation":{},"ISSN":["1747-0277","1747-0285"],"issn-type":[{"value":"1747-0277","type":"print"},{"value":"1747-0285","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,4]]},"assertion":[{"value":"2024-11-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-03-28","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-04-19","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70105"}}