{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T02:02:25Z","timestamp":1789524145815,"version":"build-2803163510"},"reference-count":34,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,6,9]],"date-time":"2025-06-09T00:00:00Z","timestamp":1749427200000},"content-version":"vor","delay-in-days":8,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100018552","name":"Science and Technology Plan Project of Taizhou","doi-asserted-by":"publisher","award":["23ywa34"],"award-info":[{"award-number":["23ywa34"]}],"id":[{"id":"10.13039\/501100018552","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chem Biol Drug Des"],"published-print":{"date-parts":[[2025,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Ulcerative colitis (UC) is a chronic inflammatory condition. Glutamine (Gln) has shown an improved effect on UC. However, its molecular determinants are incompletely understood. NCM460 cells were stimulated with lipopolysaccharide (LPS) to generate an in\u00a0vitro UC cell model, and dextran sulfate sodium (DSS)\u2010induced UC models were established in mice. Methylated RNA immunoprecipitation (MeRIP) and messenger RNA (mRNA) stability experiments were used to validate the influence of Wilms' tumor 1\u2010associating protein (WTAP) on nuclear receptor coactivator\u20103 (NCOA3) mRNA. In LPS\u2010exposed NCM460 cells, Gln promoted NCOA3 expression and reduced WTAP expression. Gln relieved LPS\u2010triggered inflammation, oxidative stress, and apoptosis in NCM460 cells, which were abolished by NCOA3 downregulation or WTAP upregulation. Mechanistically, Gln suppressed WTAP\u2010mediated m6A modification of NCOA3 mRNA. WTAP reduction attenuated LPS\u2010evoked NCM460 cell phenotype alterations, which were reversed by NCOA3 downregulation. Furthermore, Gln reduced the DAI score and histopathological changes, increased colon length, and attenuated inflammation and oxidative stress in DSS\u2010induced UC mice, which were abrogated by WTAP increase. We showed that the WTAP\/NCOA3 axis underlies the protective effect of Gln on UC, providing a rationale for Gln as a promising anti\u2010UC agent.<\/jats:p>","DOI":"10.1111\/cbdd.70110","type":"journal-article","created":{"date-parts":[[2025,6,12]],"date-time":"2025-06-12T10:51:44Z","timestamp":1749725504000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Glutamine Attenuates Inflammation and Oxidative Stress in Ulcerative Colitis by Suppressing Wilms' Tumor 1 Associated Protein\u2010Mediated <scp>N6<\/scp>\u2010Methyladenosine Modification of Nuclear Receptor Coactivator 3"],"prefix":"10.1111","volume":"105","author":[{"given":"Liangxian","family":"Jiang","sequence":"first","affiliation":[{"name":"Department of Gastrointestinal Surgery Taizhou Hospital of Zhejiang Province  Taizhou City Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuai","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Gastrointestinal Surgery Taizhou Hospital of Zhejiang Province  Taizhou City Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feifei","family":"Ye","sequence":"additional","affiliation":[{"name":"Department of Gastrointestinal Surgery Taizhou Hospital of Zhejiang Province  Taizhou City Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaowei","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Gynaecology Taizhou Hospital of Zhejiang Province  Taizhou City Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,6,9]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/jlb.4a0122\u2010068r"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.7150\/ijbs.74864"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.7150\/ijbs.28576"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu10111564"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10753\u2010020\u201001361\u20103"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010023\u201039173\u20102"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.1079011"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1155\/2022\/7280977"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms25137037"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2018.11.033"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu10030288"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392\u2010020\u201000450\u2010x"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2023.1250218"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms18051051"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1186\/s11658\u2010022\u201000350\u20108"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0140\u20106736(23)00966\u20102"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00109\u2010022\u201002231\u20101"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2023.110832"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010022\u201032163\u2010w"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13062\u2010023\u201000395\u20105"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12020\u2010021\u201002819\u20106"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsinfecdis.3c00014"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010021\u201082945\u20103"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu15245049"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2023.1217165"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cyto.2022.156074"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2023.23814"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12876\u2010023\u201002641\u20106"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaut.2024.103295"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.22038\/ijbms.2020.39815.9436"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41420\u2010022\u201000849\u20101"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.2174\/1570180819666220509070003"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2023.155153"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10753\u2010020\u201001409\u20104"}],"container-title":["Chemical Biology &amp; 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