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It is represented by over 250 species, many of which have been used as folk medicine. This study aims to determine the cholinesterase inhibitory potential of secondary metabolites of\n                    <jats:italic>Verbascum uschakense<\/jats:italic>\n                    (Murb.) Hub.\u2010Mor, an endemic species to T\u00fcrkiye that has not been studied phytochemically before. Gluroside, an iridoid glucoside, was isolated from\n                    <jats:italic>V. uschakense<\/jats:italic>\n                    through acetylcholinesterase (AChE) inhibitory activity\u2010guided fractionation alongside four other iridoid glucosides: ajugol, harpagide, aucubin, and catalpol. Additionally, three phenylethanoid glycosides\u2014verbascoside, martinoside, and forsythoside B\u2014were isolated from the antioxidant fractions evaluated using DPPH radical scavenging activity. Gluroside emerged as the most active compound against butyrylcholinesterase (BChE) with an IC\n                    <jats:sub>50<\/jats:sub>\n                    of 2.5\u2009\u00b1\u20090.02\u2009\u03bcg\/mL, exhibiting selectivity over AChE (37.69% inhibition at 200\u2009\u03bcg\/mL). Molecular modeling predicted strong electrostatic interactions between the glucosides and the catalytic residues of BChE. This is the first report of the isolation of gluroside from a\n                    <jats:italic>Verbascum<\/jats:italic>\n                    species and its cholinesterase inhibitory activity, underpinning the importance of\n                    <jats:italic>V. uschakense<\/jats:italic>\n                    and its secondary metabolites as a new class of cholinesterase inhibitors.\n                  <\/jats:p>","DOI":"10.1111\/cbdd.70178","type":"journal-article","created":{"date-parts":[[2025,10,3]],"date-time":"2025-10-03T09:25:28Z","timestamp":1759483528000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Unveiling a New Cholinesterase Inhibitor Iridoid From\n                    <i>Verbascum uschakense<\/i>\n                    (Murb.) Hub.\u2010Mor.: In\u00a0Vitro and\n                    <i>In Silico<\/i>\n                    Evaluation"],"prefix":"10.1111","volume":"106","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1596-2897","authenticated-orcid":false,"given":"Rabia Sena","family":"M\u0131nd\u0131z","sequence":"first","affiliation":[{"name":"Department of Pharmacognosy Faculty of Pharmacy, Hacettepe University  Ankara T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7946-6545","authenticated-orcid":false,"given":"G\u00fcla\u00e7t\u0131","family":"Top\u00e7u","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy Faculty of Pharmacy, Bezmialem Vak\u0131f University  \u0130stanbul T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8264-5544","authenticated-orcid":false,"given":"Didem","family":"\u015e\u00f6hreto\u011flu","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy Faculty of Pharmacy, Hacettepe University  Ankara T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8248-4218","authenticated-orcid":false,"given":"Suat","family":"Sari","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry Faculty of Pharmacy, Hacettepe University  Ankara T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2342-4143","authenticated-orcid":false,"given":"Mehmet Ufuk","family":"\u00d6zbek","sequence":"additional","affiliation":[{"name":"Department of Biology Faculty of Science, Gazi University  Ankara T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8096-0738","authenticated-orcid":false,"given":"\u00c7i\u011fdem","family":"Kahraman","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy Faculty of Pharmacy, Hacettepe University  Ankara T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,10,3]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11101\u2010014\u20109361\u20105"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/28.1.235"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/qua.24481"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1021\/ar100014h"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0031\u20109422(92)83740\u2010P"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/ddc3010011"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2019.02.021"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep42717"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0031\u20109422(93)85028\u2010P"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/ph16010095"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.7548"},{"issue":"1","key":"e_1_2_8_13_1","first-page":"51","article-title":"Constituents of Plants Growing in Qatar: Part xxviii. 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