{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T20:04:32Z","timestamp":1787169872012,"version":"build-2736575974"},"reference-count":41,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,10,17]],"date-time":"2025-10-17T00:00:00Z","timestamp":1760659200000},"content-version":"vor","delay-in-days":16,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004772","name":"Natural Science Foundation of Ningxia Province","doi-asserted-by":"publisher","award":["2023AAC03587"],"award-info":[{"award-number":["2023AAC03587"]}],"id":[{"id":"10.13039\/501100004772","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004772","name":"Natural Science Foundation of Ningxia Province","doi-asserted-by":"publisher","award":["2024AAC03599"],"award-info":[{"award-number":["2024AAC03599"]}],"id":[{"id":"10.13039\/501100004772","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chem Biol Drug Des"],"published-print":{"date-parts":[[2025,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Dl\u20103\u2010n\u2010Butylphthalide (NBP) has been reported to relieve neuronal damage by suppressing excessive autophagy. This study explored whether NBP could protect neurons after spinal cord injury (SCI) by inhibiting excessive autophagy, and its intrinsic mechanism were investigated. In methodology, this paper detected the Hippo\/YAP pathway activity and autophagy in peripheral blood of SCI patients. A SCI cell model and a rat SCI model were established. Through a series of assays and experimental validation, the effect and mechanism of NBP on neuronal damage after SCI were analyzed. As a result, the activated Hippo pathway, decreased YAP protein, and increased p\u2010YAP, Beclin 1, and LC3 II\/I proteins were monitored in peripheral blood of SCI patients. OGD treatment enhanced apoptosis and autophagy, activated the Hippo\/YAP pathway, and enhanced YAP nuclear translocation in PC12 cells. NBP treatment eliminated these effects of OGD on PC12 cells. YAP silencing reversed the suppression of NBP on the OGD\u2010induced PC12 cell apoptosis and autophagy. In\u00a0vivo, the inhibition of NBP on neuronal injury, the Hippo\/YAP pathway activity, and autophagy was abolished by YAP silencing. Thus, NBP attenuates autophagy to alleviate neuronal apoptosis after SCI via inactivating the Hippo\/YAP pathway. NBP may be useful in SCI treatment clinically.<\/jats:p>","DOI":"10.1111\/cbdd.70180","type":"journal-article","created":{"date-parts":[[2025,10,17]],"date-time":"2025-10-17T10:21:32Z","timestamp":1760696492000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Dl\u20103\u2010n\u2010Butylphthalide Inhibits Autophagy to Alleviate the Neuronal Apoptosis After Spinal Cord Injury by Elevating\n                    <scp>YAP<\/scp>\n                    via Inactivating the Hippo Signaling Pathway"],"prefix":"10.1111","volume":"106","author":[{"given":"Shengsen","family":"Yang","sequence":"first","affiliation":[{"name":"Department of Orthopaedics General Hospital of Ningxia Medical University  Yinchuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenbo","family":"Gu","sequence":"additional","affiliation":[{"name":"Ningxia Medical University  Yinchuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhongtang","family":"Ma","sequence":"additional","affiliation":[{"name":"Hongsipu District People's Hospital of Ningxia  Hongsipu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruobing","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Pharmacy General Hospital of Ningxia Medical University  Yinchuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,10,17]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2021.646788"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.2147\/DDDT.S189472"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2021.04.006"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1161\/JAHA.115.001770"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainresbull.2021.10.017"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23073728"},{"issue":"3","key":"e_1_2_9_8_1","first-page":"1075","article-title":"Dl\u20103\u2010n\u2010Butylphthalide Improves Functional Recovery in Rats With Spinal Cord Injury by Inhibiting Endoplasmic Reticulum Stress\u2010Induced Apoptosis","volume":"9","author":"He Z.","year":"2017","journal-title":"American Journal of Translational Research"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.13212"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2021.656647"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419\u2010021\u201003722\u20108"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.3969\/j.issn.1673\u20105374.2013.24.007"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2007.12.018"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.3109\/00207454.2013.864289"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2021.761273"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13287\u2010020\u201002090\u2010y"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2020.112583"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/aps.2012.35"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/4343084"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/bs.irn.2019.06.009"},{"issue":"6","key":"e_1_2_9_21_1","first-page":"6117","article-title":"Trem1 Mediates Neuronal Apoptosis via Interaction With SYK After Spinal Cord Ischemia\u2010Reperfusion Injury","volume":"13","author":"Shi W.","year":"2021","journal-title":"American Journal of Translational Research"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1080\/15384101.2021.1962634"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers10040094"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1097\/BRS.0000000000003503"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.2174\/1871527317666180612125843"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12974\u2010021\u201002312\u2010x"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.3892\/etm.2021.9786"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.14691"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.16220"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2229\u201019.2020"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12964\u2010023\u201001457\u2010x"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11596\u2010020\u20102286\u2010x"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11064\u2010017\u20102328\u20103"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010018\u201004620\u2010y"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jot.2020.02.011"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1155\/2019\/1642575"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1111\/jnc.14154"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jphotobiol.2018.05.010"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11064\u2010021\u201003361\u20107"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.7150\/ijbs.21107"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12935\u2010019\u20100898\u20107"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neulet.2020.134937"}],"container-title":["Chemical Biology &amp; Drug Design"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cbdd.70180","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T13:19:10Z","timestamp":1761657550000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/cbdd.70180"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10]]},"references-count":41,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2025,10]]}},"alternative-id":["10.1111\/cbdd.70180"],"URL":"https:\/\/doi.org\/10.1111\/cbdd.70180","archive":["Portico"],"relation":{},"ISSN":["1747-0277","1747-0285"],"issn-type":[{"value":"1747-0277","type":"print"},{"value":"1747-0285","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,10]]},"assertion":[{"value":"2024-03-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-09-24","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-10-17","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70180"}}