{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T13:23:59Z","timestamp":1761657839125,"version":"build-2065373602"},"reference-count":46,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T00:00:00Z","timestamp":1761004800000},"content-version":"vor","delay-in-days":20,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chem Biol Drug Des"],"published-print":{"date-parts":[[2025,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Primary Sjogren's syndrome (pSS) presents as a persistent inflammatory condition marked by a spectrum of symptoms and a limited array of conventional therapeutic interventions. Within Traditional Chinese Medicine (TCM), the Qiju Dihuang Pill (QJDHW) stands as a frequently employed prescription for addressing this syndrome, yet the underlying therapeutic mechanisms remain elusive. Traditional Chinese Medicine Systems Pharmacology (TCMSP) and three disease gene databases, DisGnet, GeneCards, and OMIM, were utilized to establish QJDHW targets and pSS\u2010related gene sets. Cytoscape was used to construct Protein\u2013protein interaction (PPI) and active compound\u2010target networks, followed by Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment via the DAVID database. Molecular docking was conducted with AutoDock Vina, and in\u00a0vitro experiments verified the key findings. A total of 62 overlapping targets were identified. Network analysis revealed quercetin and kaempferol as major active compounds. KEGG enrichment indicated that QJDHW may exert its therapeutic effects by modulating the immune\u2010inflammatory response. Four key targets, namely NFKBIA, IL\u20106, JUN, and IL\u20101B, were identified as the central mediators. Molecular docking analysis confirmed their binding affinity with the major active compounds. In\u00a0vitro assays further confirmed that quercetin and kaempferol suppressed the mRNA expression of key inflammation\u2010related factors, including IL\u20106, IL\u20101B, JUN, and NFKB, while reducing IL\u20106 and IL\u20101B protein levels and significantly inhibiting the phosphorylation of JUN and NFKB. Collectively, the integration of network pharmacology and experimental validation demonstrated that QJDHW exerts anti\u2010inflammatory effects in treating pSS.<\/jats:p>","DOI":"10.1111\/cbdd.70186","type":"journal-article","created":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T05:20:39Z","timestamp":1761110439000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Potential Mechanism of Qiju Dihuang Pill in Treating Primary Sjogren's Syndrome Revealed by Network Pharmacology and Experimental Verification"],"prefix":"10.1111","volume":"106","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-3061-0964","authenticated-orcid":false,"given":"Huiying","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Rheumatology, Huzhou Central Hospital Affiliated Central Hospital Huzhou University  Huzhou China"},{"name":"Wenzhou Medical University  Wenzhou 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