{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T11:36:30Z","timestamp":1790422590252,"version":"4.1.0"},"reference-count":40,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,12,17]],"date-time":"2025-12-17T00:00:00Z","timestamp":1765929600000},"content-version":"vor","delay-in-days":16,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chem Biol Drug Des"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>This study investigates whether sevoflurane postconditioning (SpostC) alleviates myocardial microcirculatory reperfusion injury by regulating mitophagy via the PTEN\u2010induced kinase 1 (PINK1)\/Parkin pathway. A mouse model of myocardial ischemia\/reperfusion (I\/R) injury was established. Microvascular perfusion was assessed by immunofluorescence. Protein expression of endothelial nitric oxide synthase (eNOS), phosphorylated eNOS (p\u2010eNOS), endothelin\u20101 (ET\u20101), PINK1, Parkin, microtubule\u2010associated protein 1A\/1B\u2010light chain 3 (LC3)II\/LC3I, and P62 was analyzed by Western blotting (WB). Hematoxylin\u2013eosin and 2,3,5\u2010triphenyltetrazolium chloride (TTC) staining were used to evaluate vascular pathology and infarct size. In\u00a0vitro, human cardiac microvascular endothelial cells (hCMECs) were subjected to oxygen\u2013glucose deprivation\/reoxygenation (OGD\/R), with assessments of cell viability (cell counting kit\u20108), barrier function (FITC\u2010dextran permeability and transendothelial electrical resistance), cytoskeletal integrity (immunofluorescence), and mitophagy markers (WB). SpostC enhanced microvascular perfusion, reduced infarct size, suppressed excessive mitophagy, and restored endothelial function. These protective effects were reversed by either the mitophagy inducer carbonyl cyanide m\u2010chlorophenylhydrazone (CCCP) or PINK1 overexpression. SpostC exerts protective effects on myocardial microcirculatory reperfusion injury by inhibiting the PINK1\/Parkin\u2010mediated mitophagy pathway.<\/jats:p>","DOI":"10.1111\/cbdd.70228","type":"journal-article","created":{"date-parts":[[2025,12,17]],"date-time":"2025-12-17T18:25:21Z","timestamp":1765995921000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Sevoflurane Postconditioning Regulates Mitophagy Through the\n                    <scp>PINK1<\/scp>\n                    \/Parkin Pathway to Alleviate Myocardial Microcirculatory Reperfusion Injury"],"prefix":"10.1111","volume":"106","author":[{"given":"Yanming","family":"Xue","sequence":"first","affiliation":[{"name":"Department of Anesthesiology Yantaishan Hospital Affiliated to Binzhou Medical University  Yantai Shandong 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