{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T20:20:01Z","timestamp":1792182001370,"version":"4.4.0"},"reference-count":29,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T00:00:00Z","timestamp":1769558400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T00:00:00Z","timestamp":1769558400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chem Biol Drug Des"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Problems such as high toxicity, low selectivity, and drug resistance encountered in cancer treatment increase the need for more effective and safe anticancer agents. In this study, the anticancer effects of new compounds consisting of thiazole and 1,3,4\u2010thiadiazole derivatives were investigated. Compound\n                    <jats:bold>6e<\/jats:bold>\n                    , which showed the strongest cytotoxic effect, was evaluated in detail in human bladder (HTB\u20109) and lung (A549) cancer cells. According to the WST\u20101 test results, the IC\n                    <jats:sub>50<\/jats:sub>\n                    value of compound\n                    <jats:bold>6e<\/jats:bold>\n                    was determined as 38.9\u2009\u03bcM for HTB\u20109 cells and 82.86\u2009\u03bcM for A549 cells. As a result of Annexin V\u2010FITC\/PI double staining and flow cytometry analysis, it was determined that compound\n                    <jats:bold>6e<\/jats:bold>\n                    application significantly increased the apoptotic cell rates in both HTB\u20109 and A549 cells. It was observed that it significantly induced early apoptosis in HTB\u20109 cells and late apoptosis in A549 cells (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.0001). Caspase\u20103\/7 activity was significantly increased in HTB\u20109 cells after compound\n                    <jats:bold>6e<\/jats:bold>\n                    application compared to the control group and similarly increased in A549 cells (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.0001). In cell cycle analyses, compound\n                    <jats:bold>6e<\/jats:bold>\n                    caused cell cycle arrest in the G2\/M phase in HTB\u20109 and A549 cells. In the wound healing test, the migration speed in HTB\u20109 and A549 cells treated with compound\n                    <jats:bold>6e<\/jats:bold>\n                    after 24\u2009h was significantly decreased (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.0001). Molecular docking revealed strong binding to caspase\u20103 (PDB ID: 1NMQ, score: \u22128.2\u2009kcal\/mol), identifying key interactions that mechanistically support its pro\u2010apoptotic effects. In addition, the low cytotoxicity of compound\n                    <jats:bold>6e<\/jats:bold>\n                    in healthy BEAS\u20102B cells suggests that it has a selective anticancer effect.\n                  <\/jats:p>","DOI":"10.1111\/cbdd.70246","type":"journal-article","created":{"date-parts":[[2026,1,29]],"date-time":"2026-01-29T04:10:39Z","timestamp":1769659839000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Thiazole\u2010 and 1,3,4\u2010Thiadiazole\u2010Based Hybrids: Synthesis and In\u00a0Vitro Cytotoxicity Against Human Cancer Cell Lines"],"prefix":"10.1111","volume":"107","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9106-3848","authenticated-orcid":false,"given":"Emine","family":"Terzi","sequence":"first","affiliation":[{"name":"Department of Medical Biology, Faculty of Medicine Ankara Yildirim Beyazit University  Ankara T\u00fcrkiye"},{"name":"Yenimahalle Training and Research Hospital Ankara Yildirim Beyazit University  Ankara T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9468-4719","authenticated-orcid":false,"given":"Tuba Ozdemir","family":"Sanci","sequence":"additional","affiliation":[{"name":"Yenimahalle Training and Research Hospital Ankara Yildirim Beyazit University  Ankara T\u00fcrkiye"},{"name":"Department of Histology and Embryology, Faculty of Medicine Ankara Yildirim Beyazit University  Ankara T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0596-834X","authenticated-orcid":false,"given":"Beyza Ecem Oz","family":"Bedir","sequence":"additional","affiliation":[{"name":"Department of Medical Biology, Faculty of Medicine Ankara Yildirim Beyazit University  Ankara T\u00fcrkiye"},{"name":"Yenimahalle Training and Research Hospital Ankara Yildirim Beyazit University  Ankara T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8120-5849","authenticated-orcid":false,"given":"Elif","family":"Ercan","sequence":"additional","affiliation":[{"name":"Department of Medical Biology, Faculty of Medicine Ankara Yildirim Beyazit University  Ankara T\u00fcrkiye"},{"name":"Yenimahalle Training and Research Hospital Ankara Yildirim Beyazit University  Ankara T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emine Rana","family":"Ba\u011fc\u0131","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry, Faculty of Pharmacy Afyonkarahisar Health Sciences University  Afyonkarahisar Turkey"},{"name":"Department of Pharmaceutical Chemistry Graduate School, Anadolu University  Eski\u015fehir T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1879-1034","authenticated-orcid":false,"given":"Ulviye Acar","family":"\u00c7evik","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry, Faculty of Pharmacy Anadolu University  Eski\u015fehir T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ecem Kaya","family":"Sezginer","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Pharmacy Ankara University  Ankara 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