{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,12]],"date-time":"2026-04-12T12:45:08Z","timestamp":1775997908126,"version":"3.50.1"},"reference-count":25,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,1,31]],"date-time":"2026-01-31T00:00:00Z","timestamp":1769817600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,1,31]],"date-time":"2026-01-31T00:00:00Z","timestamp":1769817600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100010814","name":"Anhui Provincial Department of Education","doi-asserted-by":"publisher","award":["2024AH051980"],"award-info":[{"award-number":["2024AH051980"]}],"id":[{"id":"10.13039\/501100010814","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chem Biol Drug Des"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Twenty\u2010six novel derivatives of paeonol chalcone were synthesized by introducing various hydrophilic aminoalkyl to the 2\u2032\u2010hydroxy of paeonol. Their antiproliferative activity was evaluated by MTT assay against five human cancer cell lines (HeLa, HepG2, U2OS, HCT\u2010116 and A549). The majority of these compounds demonstrated remarkable cytotoxicity against the tested cancer cells. Among them, compound\n                    <jats:bold>4k<\/jats:bold>\n                    exhibited the most potent antiproliferative activity (IC\n                    <jats:sub>50<\/jats:sub>\n                    values ranging from 1.31\u2009\u03bcM to 7.56\u2009\u03bcM for the tested cancer cells), stronger than 5\u2010FU (IC\n                    <jats:sub>50<\/jats:sub>\n                    \u2009=\u20096.29\u201314.56\u2009\u03bcM). Notably, it effectively inhibited the proliferation of HeLa\/Taxol cells (IC\n                    <jats:sub>50<\/jats:sub>\n                    \u2009=\u200914.47\u2009\u00b1\u20090.02\u2009\u03bcM) while showing low cytotoxicity toward normal liver epithelial THLE\u20102 cells (IC\n                    <jats:sub>50<\/jats:sub>\n                    \u2009=\u200926.40\u2009\u00b1\u20090.04\u2009\u03bcM). Preliminary mechanistic studies revealed that compound\n                    <jats:bold>4k<\/jats:bold>\n                    induces apoptosis in HeLa cells and downregulates the expression of Cyclin B1 and CDK1, leading to cell cycle arrest at the G2\/M transition. Solubility studies revealed that\n                    <jats:bold>5k<\/jats:bold>\n                    (\n                    <jats:bold>4k<\/jats:bold>\n                    hydrochloride) showed excellent aqueous solubility (11.53\u2009mg\/mL). SwissADME predictions further supported its potential druggability. Collectively, compound\n                    <jats:bold>4k<\/jats:bold>\n                    represents a promising lead worthy of further investigation as a potential antitumor agent.\n                  <\/jats:p>","DOI":"10.1111\/cbdd.70255","type":"journal-article","created":{"date-parts":[[2026,1,31]],"date-time":"2026-01-31T10:18:23Z","timestamp":1769854703000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Design, Synthesis and Biological Evaluation of Aminoalkylated Paeonol Chalcone Derivatives"],"prefix":"10.1111","volume":"107","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-9545-9521","authenticated-orcid":false,"given":"Jia\u2010chen","family":"Xu","sequence":"first","affiliation":[{"name":"College of Pharmacy Anhui University of Chinese Medicine  Hefei China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bei\u2010bei","family":"Feng","sequence":"additional","affiliation":[{"name":"College of Pharmacy Anhui University of Chinese Medicine  Hefei China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong","family":"Wu","sequence":"additional","affiliation":[{"name":"West Anhui Health Vocational College  Luan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li\u2010qin","family":"He","sequence":"additional","affiliation":[{"name":"College of Pharmacy Anhui University of Chinese Medicine  Hefei China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peng","family":"Huang","sequence":"additional","affiliation":[{"name":"College of Pharmacy Anhui University of Chinese Medicine  Hefei China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,1,31]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioorg.2022.106171"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21834"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox14020148"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep42717"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/med.21698"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/cbdd.14389"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/cbdd.70079"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41429\u2010020\u20100280\u2010y"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1039\/c6md00252h"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00044\u2010017\u20102120\u20106"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.18632\/aging.102288"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.2174\/1568026617666170914160446"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev\u2010pathmechdis\u2010051222\u2010115023"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom11060894"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2020.118745"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10311\u2010019\u201000959\u2010w"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.2174\/0929867325666181001112226"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules26237177"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21708"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.3389\/fchem.2021.766201"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10495\u2010024\u201002038\u20100"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2018.11.019"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2021.113897"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12192\u2010022\u201001302\u2010z"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2017.12.053"}],"container-title":["Chemical Biology &amp; 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