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Building on our previous work with 3\u2010substituted coumarins as inhibitors of tumor\u2010associated carbonic anhydrases, we report a novel series of thiazol\u2010hydrazono\u2010coumarins targeting the ATP\u2010binding domain of topoisomerase enzymes. Seventeen compounds were synthesized and evaluated for selective cytotoxicity against HeLa cells versus WI\u201038 fibroblasts and for antimicrobial activity against four ESKAPE pathogens,\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Escherichia coli<\/jats:italic>\n                    <\/jats:styled-content>\n                    , and\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Salmonella typhimurium<\/jats:italic>\n                    <\/jats:styled-content>\n                    . Several derivatives showed potent antibacterial activity, with MICs as low as 0.12\u2009\u03bcg\/mL against resistant\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    <\/jats:styled-content>\n                    strains and inhibition zones up to 33\u2009mm against Gram\u2010negative bacteria. Compound 13 exhibited strong selectivity, with an IC\n                    <jats:sub>50<\/jats:sub>\n                    of 26.8\u2009\u03bcg\/mL in HeLa cells and 220.7\u2009\u03bcg\/mL in WI\u201038 cells. The five most active compounds were studied via molecular docking and MM\/GBSA to elucidate their binding to bacterial DNA gyrase, topoisomerase IV, and human topoisomerase II\u03b1. A molecular dynamics simulation of the\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>S. aureus<\/jats:italic>\n                    <\/jats:styled-content>\n                    DNA gyrase B\u2010compound 13 complex revealed a novel hydrogen bond between the coumarin ring and serine\u2010129. These findings highlight thiazol\u2010hydrazono\u2010coumarins as promising antibacterial leads with ancillary anticancer activity, supporting their potential in treating infections in immunocompromised cancer patients.\n                  <\/jats:p>","DOI":"10.1111\/cbdd.70261","type":"journal-article","created":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T05:01:10Z","timestamp":1771650070000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Coumarin\u2010Augmented Thiazole Hybrids as Dual Anticancer and Antibacterial Agents"],"prefix":"10.1111","volume":"107","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5997-2574","authenticated-orcid":false,"given":"Islam K.","family":"Matar","sequence":"first","affiliation":[{"name":"Department of Chemistry Saint Mary's University  Halifax Nova Scotia Canada"},{"name":"Department of Chemistry and Physics Mount Saint Vincent University  Halifax Nova Scotia Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5643-9202","authenticated-orcid":false,"given":"Magdi E. 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