{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,26]],"date-time":"2026-05-26T19:03:03Z","timestamp":1779822183289,"version":"3.53.1"},"reference-count":40,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T00:00:00Z","timestamp":1778716800000},"content-version":"vor","delay-in-days":13,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chem Biol Drug Des"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>tRNA\u2010derived small RNAs (tsRNAs) are small single\u2010stranded RNAs cleaved from precursor and mature tRNAs. Aberrant expression of tsRNAs has been reported in multiple cancers, suggesting their potential as novel biomarkers and therapeutic targets. However, the role of tsRNAs in nasopharyngeal carcinoma (NPC) remains unclear. This study aimed to investigate tsRNA expression profiles in serum exosomes of NPC and explore the function of dysregulated tsRNAs. Serum samples from 30 NPC patients and 30 healthy controls, as well as 10 pairs of NPC tumor and adjacent normal tissues were collected. Serum exosomes were isolated by ultracentrifugation and identified using transmission electron microscopy. Exosomal tsRNA expression was analyzed by high\u2010throughput sequencing in 3 paired samples. Dysregulated tsRNAs were validated by RT\u2010qPCR in NPC cell lines (5\u20108F, CNE1, CNE2), NP69 cells, clinical serum exosomes and tissues. ROC curves were used to evaluate diagnostic values. CCK\u20108, EdU, colony formation and Transwell assays were performed to assess cell functions. GO and KEGG enrichment analyses were conducted to functionally annotate target genes and identify significantly enriched biological pathways. The expression of 247 tsRNAs varied significantly in serum exosomes, including 142 upregulated and 105 down\u2010regulated, in NPC patients compared with healthy controls. RT\u2010qPCR validation demonstrated that the expression levels of tRNA\u2010Lys\u2010CTT\u20101\u2010M5 and tRNA\u2010Thr\u2010TGT\u20102 were significantly upregulated in NPC cells, tumor tissues, and serum exosomes compared with their normal nasopharyngeal cell, tissue, and serum exosome counterparts from healthy individuals. And in the diagnosis of NPC, the AUC values of exosomal tRNA\u2010Lys\u2010CTT\u20101\u2010M5 and tRNA\u2010Thr\u2010TGT\u20102 based on ROC curve analysis were 0.88 and 0.81, respectively. Functional experiments showed that tRNA\u2010Lys\u2010CTT\u20101\u2010M5 and tRNA\u2010Thr\u2010TGT\u20102 promoted NPC cell proliferation, while tRNA\u2010Thr\u2010TGT\u20102 enhanced cell migration. These results indicate that tRNA\u2010Thr\u2010TGT\u20102 plays an important role in the pathogenesis of nasopharyngeal carcinoma (NPC) and has the potential to serve as a novel diagnostic biomarker. Furthermore, tRNA\u2010Thr\u2010TGT\u20102 is expected to become a promising therapeutic target for the treatment of NPC.<\/jats:p>","DOI":"10.1111\/cbdd.70313","type":"journal-article","created":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T11:52:36Z","timestamp":1778759556000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Multi\u2010Omics Analysis and Experimental Verification Reveal Multiple Roles of tRNA\u2010Derived Fragments in Nasopharyngeal Carcinoma"],"prefix":"10.1111","volume":"107","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-9622-2405","authenticated-orcid":false,"given":"Liang","family":"Gu","sequence":"first","affiliation":[{"name":"Department of Radiotherapy The Affiliated Cancer Hospital of Nanjing Medical University &amp; Jiangsu Cancer Hospital &amp; Jiangsu Institute of Cancer Research  Nanjing Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tianye","family":"Qian","sequence":"additional","affiliation":[{"name":"Department of Gynecologic Oncology The Affiliated Cancer Hospital of Nanjing Medical University &amp; Jiangsu Cancer Hospital &amp; Jiangsu Institute of Cancer Research  Nanjing Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Radiotherapy The Affiliated Cancer Hospital of Nanjing Medical University &amp; Jiangsu Cancer Hospital &amp; Jiangsu Institute of Cancer Research  Nanjing Jiangsu China"},{"name":"Department of Environmental Genomics, Jiangsu Key Laboratory of Cancer Biomarkers, Prevention and Treatment, Collaborative Innovation Center for Cancer Personalized Medicine Nanjing Medical University  Nanjing Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-1846-6566","authenticated-orcid":false,"given":"Yizhi","family":"Ge","sequence":"additional","affiliation":[{"name":"Department of Radiotherapy The Affiliated Cancer Hospital of Nanjing Medical University &amp; Jiangsu Cancer Hospital &amp; Jiangsu Institute of Cancer Research  Nanjing Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7927-5215","authenticated-orcid":false,"given":"Dan","family":"Zong","sequence":"additional","affiliation":[{"name":"Department of Radiotherapy The Affiliated Cancer Hospital of Nanjing Medical University &amp; Jiangsu Cancer Hospital &amp; Jiangsu Institute of Cancer Research  Nanjing Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xia","family":"He","sequence":"additional","affiliation":[{"name":"Department of Radiotherapy The Affiliated Cancer Hospital of Nanjing Medical University &amp; Jiangsu Cancer Hospital &amp; Jiangsu Institute of Cancer Research  Nanjing Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,5,14]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2024.1392245"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev\u2010biochem\u2010060815\u2010014710"},{"key":"e_1_2_13_4_1","doi-asserted-by":"publisher","DOI":"10.1080\/17501911.2026.2642583"},{"key":"e_1_2_13_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbc.2023.104966"},{"key":"e_1_2_13_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers16050919"},{"key":"e_1_2_13_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2022.11.295"},{"key":"e_1_2_13_8_1","doi-asserted-by":"publisher","DOI":"10.4067\/s0034\u201098872023000901221"},{"key":"e_1_2_13_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12943\u2010023\u201001739\u20105"},{"key":"e_1_2_13_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11912\u2010019\u20100846\u20107"},{"key":"e_1_2_13_11_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12935\u2010021\u201002418\u20105"},{"key":"e_1_2_13_12_1","doi-asserted-by":"publisher","DOI":"10.21037\/tlcr-21-829"},{"key":"e_1_2_13_13_1","doi-asserted-by":"publisher","DOI":"10.3892\/ijo.2023.5545"},{"key":"e_1_2_13_14_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13058\u2010024\u201001870\u20101"},{"key":"e_1_2_13_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2022.05.027"},{"key":"e_1_2_13_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/s1470\u20102045(05)70168\u20106"},{"key":"e_1_2_13_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature25005"},{"key":"e_1_2_13_18_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24119559"},{"key":"e_1_2_13_19_1","doi-asserted-by":"publisher","DOI":"10.1172\/jci148130"},{"key":"e_1_2_13_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkac1018"},{"key":"e_1_2_13_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2024.122903"},{"key":"e_1_2_13_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.35041"},{"key":"e_1_2_13_23_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev\u2010biochem\u2010013118\u2010111902"},{"key":"e_1_2_13_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2019.05.007"},{"key":"e_1_2_13_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/ajg.2017.38"},{"key":"e_1_2_13_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijrobp.2026.03.008"},{"key":"e_1_2_13_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2025.07.019"},{"key":"e_1_2_13_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2024.111511"},{"key":"e_1_2_13_29_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers16081588"},{"key":"e_1_2_13_30_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers15030899"},{"key":"e_1_2_13_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.omto.2022.02.008"},{"key":"e_1_2_13_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijscr.2025.111073"},{"key":"e_1_2_13_33_1","doi-asserted-by":"publisher","DOI":"10.32604\/or.2026.075012"},{"key":"e_1_2_13_34_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24010322"},{"key":"e_1_2_13_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2025.153211"},{"key":"e_1_2_13_36_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12935\u2010021\u201002102\u20108"},{"key":"e_1_2_13_37_1","doi-asserted-by":"publisher","DOI":"10.7150\/jca.22505"},{"key":"e_1_2_13_38_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12079\u2010022\u201000690\u20102"},{"key":"e_1_2_13_39_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12967\u2010025\u201006109\u2010x"},{"key":"e_1_2_13_40_1","doi-asserted-by":"publisher","DOI":"10.1080\/15384101.2023.2216508"},{"key":"e_1_2_13_41_1","doi-asserted-by":"publisher","DOI":"10.1109\/rbme.2023.3269776"}],"container-title":["Chemical Biology &amp; 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