{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T18:17:19Z","timestamp":1788891439997,"version":"build-2803163510"},"reference-count":61,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,5,24]],"date-time":"2026-05-24T00:00:00Z","timestamp":1779580800000},"content-version":"vor","delay-in-days":23,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chem Biol Drug Des"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Gestational diabetes mellitus (GDM) is a complex metabolic disorder characterized by glucose intolerance during pregnancy, posing significant health risks to both the mother and fetus. Current therapeutic options, including Metformin, show limitations, highlighting the need for novel, or adjunctive treatments to improve outcomes. This study aimed to identify novel and adjunctive phytocompounds with therapeutic potential against GDM using computational and experimental approaches. Text mining was employed to compile a phytochemical library, followed by molecular docking using AutoDock Vina against 17 GDM\u2010associated targets. Drug\u2010likeness, pharmacophore scoring, ADMET screening, molecular dynamics simulation, and in\u00a0vitro studies were conducted to prioritize potent candidates. Berberine emerged as a promising anti\u2010GDM compound based on docking scores, binding energies, and hydrogen bond interactions with GDM receptors. It achieved the highest pharmacophore selectivity score of 19.71 and met all drug\u2010likeness criteria. Berberine demonstrated favorable renal excretion, high intestinal absorption (HIA) with 100% CaCO\n                    <jats:sub>2<\/jats:sub>\n                    permeability, and acceptable blood\u2013brain barrier (BBB) permeability. MD simulation revealed the high stability of Berberine. In\u00a0vitro assays confirmed high glucose uptake (40.88\u2009\u00b1\u20090.51) and increased insulin secretion (129.78\u2009\u00b1\u20091.26) in the PANC\u20101 cell line in the Berberine\u2010treated group as compared to the Metformin, revealing its superior anti\u2010diabetic activity. Further, the group treated in combination of Berberine and Metformin showed the highest glucose uptake (50.63\u2009\u00b1\u20090.47) and insulin secretion (173.66\u2009\u00b1\u20091.78). The combined computational and experimental findings suggest that Berberine, a non\u2010toxic phytochemical, holds potential as an effective therapeutic candidate for GDM. Its robust binding, high selectivity, enhanced activity and stability make it a promising lead compound for further development. Furthermore, the anti\u2010diabetic effect is augmented when Berberine and Metformin are used together, indicating the synergistic potential of Berberine and Metformin in treating hyperglycaemic conditions like GDM.\n                  <\/jats:p>","DOI":"10.1111\/cbdd.70317","type":"journal-article","created":{"date-parts":[[2026,5,25]],"date-time":"2026-05-25T06:00:14Z","timestamp":1779688814000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Synergistic Potential of Berberine and Metformin: Unlocking Enhanced Anti\u2010Diabetic Efficacy"],"prefix":"10.1111","volume":"107","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9889-2072","authenticated-orcid":false,"given":"Ravi","family":"Bhushan","sequence":"first","affiliation":[{"name":"Centre for Genetic Disorder, Institute of Science Banaras Hindu University  Varanasi Uttar Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2341-900X","authenticated-orcid":false,"given":"Md Tabish","family":"Rehman","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, College of Pharmacy King Saud University  Riyadh Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3044-1267","authenticated-orcid":false,"given":"Mohamed F.","family":"AlAjmi","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, College of Pharmacy King Saud University  Riyadh Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad Rizwan","family":"Alam","sequence":"additional","affiliation":[{"name":"Department of Hospital Pathology The Catholic University of Korea College of Medicine  Seoul Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6282-8706","authenticated-orcid":false,"given":"Safikur","family":"Rahman","sequence":"additional","affiliation":[{"name":"Department of Botany M.S. 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