{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,26]],"date-time":"2026-05-26T19:03:05Z","timestamp":1779822185662,"version":"3.53.1"},"reference-count":37,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T00:00:00Z","timestamp":1778803200000},"content-version":"vor","delay-in-days":14,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chem Biol Drug Des"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Propofol, an intravenous anesthetic, exhibits cerebroprotective effects against cerebral infarction (CI); however, its underlying mechanisms, particularly those involving ferroptosis, remain incompletely understood. This study employed oxygen\u2013glucose deprivation\/reperfusion (OGD\/R)\u2010treated SK\u2010N\u2010SH cells and middle cerebral artery occlusion (MCAO) rat models to investigate propofol's actions in\u00a0vitro and in\u00a0vivo. Propofol alleviated OGD\/R\u2010induced neuronal injury by enhancing cell viability, suppressing apoptosis, and reducing the release of inflammatory cytokines interleukin\u20106 (IL\u20106) and IL\u20101\u03b2. Importantly, propofol significantly suppressed ferroptosis, as evidenced by decreased levels of reactive oxygen species (ROS), Fe\n                    <jats:sup>2+<\/jats:sup>\n                    , and malondialdehyde (MDA), as well as restored mitochondrial membrane potential. Mechanistically, propofol up\u2010regulated the expression of tripartite motif\u2010containing 67 (TRIM67), which bound to voltage\u2010dependent anion channel 1 (VDAC1), promoting its K63\u2010linked polyubiquitination and proteasomal degradation. Conversely, VDAC1 overexpression or TRIM67 knockdown reversed the protective effects of propofol. Consistent with the in\u00a0vitro results, propofol reduced cerebral infarct volume, improved neurological function, and suppressed neuroinflammation, effects that were concomitant with increased TRIM67 and decreased VDAC1 protein expression in brain tissues. In conclusion, this study reveals the TRIM67\/VDAC1 pathway as a novel mechanism underlying propofol's cerebroprotective effects, thereby identifying a potential therapeutic target for CI.\n                  <\/jats:p>","DOI":"10.1111\/cbdd.70318","type":"journal-article","created":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T07:06:21Z","timestamp":1778828781000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Propofol Exerts Cerebroprotective Effects Against Cerebral Infarction by Up\u2010Regulating\n                    <scp>TRIM67<\/scp>\n                    to Mediate\n                    <scp>VDAC1<\/scp>\n                    Ubiquitination and Degradation"],"prefix":"10.1111","volume":"107","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-8226-8127","authenticated-orcid":false,"given":"Wang","family":"Jia","sequence":"first","affiliation":[{"name":"Department of Anesthesia Qinhuangdao City Maternal and Child Health Care Hospital  Qinhuangdao City Hebei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yinhua","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Anesthesia Qinhuangdao City Maternal and Child Health Care Hospital  Qinhuangdao City Hebei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhongyu","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Anesthesia Qinhuangdao City Maternal and Child Health Care Hospital  Qinhuangdao City Hebei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donghui","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Anesthesia The First Hospital of Qinhuangdao  Qinhuangdao City Hebei China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,5,15]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules30132795"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/pan.70001"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/aln.0000000000001534"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom15010033"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamaneurol.2023.0413"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1080\/15384047.2025.2478670"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12031\u2010020\u201001703\u20108"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11064\u2010022\u201003822\u20107"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.793147"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.3389\/fneur.2024.1332791"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1080\/14728222.2024.2446319"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2023.1161810"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419\u2010024\u201007216\u20101"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.7150\/jca.92723"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11033\u2010024\u201010123\u20105"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lddd.2025.100025"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lddd.2025.100013"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules29184359"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms26041572"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.2174\/0118761429246578231130064830"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2024.117345"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.3892\/or.2024.8814"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2025.114548"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.arr.2024.102489"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.24272\/j.issn.2095\u20108137.2024.239"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12035\u2010025\u201005220\u2010w"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13578\u2010023\u201001056\u2010w"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2025.103622"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms26083865"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12031\u2010025\u201002313\u2010y"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.3892\/ijmm.2021.5070"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41418\u2010024\u201001334\u20101"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/s43440\u2010024\u201000620\u20106"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/brb3.70187"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/cac2.70044"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2024.113468"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.curpro.2025.100016"}],"container-title":["Chemical Biology &amp; Drug Design"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cbdd.70318","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/cbdd.70318","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cbdd.70318","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,26]],"date-time":"2026-05-26T18:27:48Z","timestamp":1779820068000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/cbdd.70318"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5]]},"references-count":37,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2026,5]]}},"alternative-id":["10.1111\/cbdd.70318"],"URL":"https:\/\/doi.org\/10.1111\/cbdd.70318","archive":["Portico"],"relation":{},"ISSN":["1747-0277","1747-0285"],"issn-type":[{"value":"1747-0277","type":"print"},{"value":"1747-0285","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5]]},"assertion":[{"value":"2025-11-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-07","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-15","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70318"}}