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In this study, we sought small\u2010molecule inhibitors of LOXL2 using a library of 4\u2010thiazolidones supported by molecular docking. Five candidates were prioritised, with Les 5740 displaying the highest LOXL2 inhibitory activity (IC\n                    <jats:sub>50<\/jats:sub>\n                    of 27\u2009\u03bcM). Although Les 5740 showed low cytotoxicity in various cancer and normal\u2010like cell lines, the triple\u2010negative breast cancer (TNBC) cell line MDA\u2010MB\u2010231 showed increased susceptibility to this molecule. Les 5740 did not induce DNA damage in mammary cells. At 30\u2009\u03bcM, it increased TNBC cells' necrosis. The impact of a non\u2010toxic concentration of Les 5740 (5\u2009\u03bcM) on TNBC cell motility was evaluated. Les 5740 reduced 2D chemoinvasion and altered cell morphology. These findings are consistent with a potential reduction in extracellular matrix stiffness as a consequence of LOXL2 inhibition. Les 5740 mildly inhibited tubulin polymerisation, supporting a multi\u2010target profile. Overall, Les 5740 should be viewed as a starting scaffold with LOXL2 inhibitory activity and broader cellular effects, motivating optimisation toward more potent and selective LOXL2 inhibitors for breast cancer therapy.\n                  <\/jats:p>","DOI":"10.1111\/cbdd.70320","type":"journal-article","created":{"date-parts":[[2026,5,28]],"date-time":"2026-05-28T11:40:15Z","timestamp":1779968415000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["From Virtual Screening to Cell Phenotype: 4\u2010Thiazolidone Scaffolds Targeting\n                    <scp>LOXL2<\/scp>\n                    in Breast Cancer"],"prefix":"10.1111","volume":"107","author":[{"given":"Sandra","family":"Ferreira","sequence":"first","affiliation":[{"name":"CBIOS, Universidade Lus\u00f3fona's Research Center for Biosciences &amp; Health Technologies  Lisbon Portugal"},{"name":"Dpto. de Ciencias Biom\u00e9dicas (Farmacolog\u00eda) Universidad de Alcala  Alcal\u00e1 de Henares, Madrid Espa\u00f1a"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beatriz","family":"Santos","sequence":"additional","affiliation":[{"name":"CBIOS, Universidade Lus\u00f3fona's Research Center for Biosciences &amp; Health Technologies  Lisbon Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matilde","family":"Tavares","sequence":"additional","affiliation":[{"name":"CBIOS, Universidade Lus\u00f3fona's Research Center for Biosciences &amp; Health Technologies  Lisbon Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Serhii","family":"Holota","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical, Organic and Bioorganic Chemistry Danylo Halytsky Lviv National Medical University  Lviv Ukraine"},{"name":"Molecular Design Center Danylo Halytsky Lviv National Medical University  Lviv Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dmytro","family":"Khylyuk","sequence":"additional","affiliation":[{"name":"Department of Organic Chemistry Medical University of Lublin  Lublin Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3322-0080","authenticated-orcid":false,"given":"Roman","family":"Lesyk","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical, Organic and Bioorganic Chemistry Danylo Halytsky Lviv National Medical University  Lviv Ukraine"},{"name":"Molecular Design Center Danylo Halytsky Lviv National Medical University  Lviv Ukraine"},{"name":"Department of Biotechnology and Cell Biology University of Information Technology and Management in Rzeszow  Rzeszow Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalia","family":"Finyuk","sequence":"additional","affiliation":[{"name":"Department of Regulation of Cell Proliferation and Apoptosis Institute of Cell Biology of National Academy of Sciences of Ukraine  Lviv Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7798-8641","authenticated-orcid":false,"given":"Daniel J. 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