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Thirty\u2010three analogues of\n                    <jats:italic>N<\/jats:italic>\n                    \u2010substituted\u20107\u2010and 8\u2010methyl\u20104\u2010hydroxy\u20102\u2010quinolone\u20103\u2010carboxamides were synthesized and characterized using FT\u2010IR, NMR (\n                    <jats:sup>1<\/jats:sup>\n                    H and\n                    <jats:sup>13<\/jats:sup>\n                    C), HRMS, and elemental analysis. The analogues displayed potential cytotoxicity against human colon cancer (HCT\u2010116) and epithelial colorectal adenocarcinoma (Caco\u20102) cells. Compounds bearing a phenyl ring tailored with\n                    <jats:italic>o<\/jats:italic>\n                    \u2010CF\n                    <jats:sub>3<\/jats:sub>\n                    (\n                    <jats:bold>26<\/jats:bold>\n                    ) and a phenyl ring substituted with\n                    <jats:italic>o<\/jats:italic>\n                    \u2010COOH and\n                    <jats:italic>p<\/jats:italic>\n                    \u2010CH\n                    <jats:sub>3<\/jats:sub>\n                    (\n                    <jats:bold>29<\/jats:bold>\n                    ) exert preferential inhibitory activity against HCT\u2010116 with IC\n                    <jats:sub>50s<\/jats:sub>\n                    9.4 and 7.8\u2009\u03bcM. The bioinformatics calculations interpret the selectivity of analogues against HCT\u2010116. The principal component analysis (PCA) discloses that the analogues are regarded as lead\u2010like and nominated for hit\u2010to\u2010lead and lead optimization stages of drug discovery. Docking studies illustrate that analogues accommodate the kinase domain of PI3K\u03b1 and engage with key binding residues. The promising outcome of this work emphasizes the significance of this scaffold as anticancer agents.\n                  <\/jats:p>","DOI":"10.1111\/cbdd.70324","type":"journal-article","created":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T05:45:40Z","timestamp":1780292740000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Investigating the Anticancer Promise of Synthesized Methylated\n                    <i>N<\/i>\n                    \u2010Substituted\u20104\u2010Hydroxy\u20102\u2010Quinolone\u20103\u2010Carboxamides: Biological and Computational Characterization"],"prefix":"10.1111","volume":"107","author":[{"given":"Batool","family":"Al\u2010lahowani","sequence":"first","affiliation":[{"name":"Department of Pharmacy, Faculty of Pharmacy Al\u2010Zaytoonah University of Jordan  Amman Jordan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tahrer 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